Aloin protects against arsenic trioxide-induced myocardial membrane damage and release of inflammatory cytokines

Abstract

Aloin exerts concentration-dependent pro-oxidant and antioxidant effects when tested in vitro. Such duality of effects has not been investigated through in vivo studies on aloin. We evaluated the effects of aloin at doses ranging between 1 and 125 mg/kg against the arsenic trioxide (As₂O₃)-induced cardiotoxicity in mice. As₂O₃ (5 mg/kg/day) was intraperitoneally administrated for 10 days. Aloin was administered through oral gavage at 1, 5, 25, and 125 mg/kg/day. As₂O₃ induced rise in ST height and QT interval in ECG, increased oxidative stress, and depleted the antioxidative defense. As₂O₃ increased inflammatory cytokine concentrations in the heart. Aloin dose dependently inhibited the As₂O₃-induced cardiotoxicity. There was no evidence of increased oxidative stress in the low-dose aloin-treated mice receiving As₂O_3. Our results indicate that aloin possesses cardioprotective potentials and its pro-oxidant effect is not evident in vivo at tested doses.

Keywords: Aloin; As2O3; Myocardial damage; Oxidative stress; TNF-α.