doi: 10.1016/0022-1759(77)90004-7.
A simple method for the production of migration inhibitory factor by concanavalin A - stimulated lymphocytes
- PMID: 320265
- DOI: 10.1016/0022-1759(77)90004-7
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A simple method for the production of migration inhibitory factor by concanavalin A - stimulated lymphocytes
J Immunol Methods.
1977.
Abstract
A simple method for preparing culture supernatants containing macrophage migration inhibitory factor (MIF) by pulse exposure to guinea pig lymphocytes to concanavalin A (ConA), is described. The method is based on the property of originally nonadherent lymphocytes to attach to the flask surface in the presence of Con A. The Con A-induced lymphocyte 'monolayer' can be easily rinsed, allowing the removal of free Con A without repeated, cell damaging centrifugation. The Con A-pulsed lymphocytes are cultured for 24 h in medium and supernatants containing MIF but free of undesired Con A are regularly obtained.
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