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. 2020 Apr;9(7):2372-2378.
doi: 10.1002/cam4.2909. Epub 2020 Feb 6.

Genetic association analysis identifies a role for ANO5 in prostate cancer progression

Chia-Cheng Yu  1   2   3 , Lih-Chyang Chen  4 , Chao-Yuan Huang  5 , Victor C Lin  6   7 , Te-Ling Lu  8 , Cheng-Hsueh Lee  9 , Shu-Pin Huang  9   10   11   12 , Bo-Ying Bao  8   13   14
Affiliations

Genetic association analysis identifies a role for ANO5 in prostate cancer progression

Chia-Cheng Yu et al. Cancer Med. 2020 Apr.

Abstract

Anoctamins were originally identified as a family of calcium-activated chloride channels, but recently their roles in the development of different types of malignancies were suggested. Here, we evaluated the associations between 211 common single-nucleotide polymorphisms in 10 anoctamin genes with biochemical recurrence (BCR) after radical prostatectomy (RP) for localized prostate cancer. Four SNPs (ANO4 rs585335, ANO5 rs4622263, ANO7 rs62187431, and ANO10 rs118005571) remained significantly associated with BCR after multiple test correction (P < .05 and q = 0.232) and adjustment for known prognostic factors. Expression quantitative trait loci analysis found that ANO5 rs4622263 C and ANO10 rs118005571 C alleles were associated with decreased mRNA expression levels. Moreover, lower expression of ANO5 was correlated with more advanced tumors and poorer outcomes in two independent prostate cancer cohorts. Taken together, ANO5 rs4622263 was associated with BCR, and ANO5 gene expression was correlated with patient prognosis, suggesting a pivotal role for ANO5 in prostate cancer progression.

Keywords: anoctamin; biomarker; prognosis; progression; prostate cancer.

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Conflict of interest statement

The authors have no conflict of interest to declare.

Figures

Figure 1
Figure 1
ANO3 rs74754887, ANO4 rs585335, ANO5 rs4622263, ANO7 rs62187431, and ANO10 rs118005571 are associated with biochemical recurrence (BCR)‐free survival time. Values between brackets denote the number of patients
Figure 2
Figure 2
Functional analyses of candidate single‐nucleotide polymorphisms. A, Expression quantitative trait loci analyses identify two significant associations between rs4622263 and ANO5, and rs118005571 and ANO10 in 523 HapMap lymphoblastoid cell lines. B, Lower expression of ANO5 is correlated with prostate cancer, higher Gleason score, and stage, and shorter time to biochemical recurrence in Memorial Sloan‐Kettering Cancer Center cohort. C, Expression of ANO10 is not altered over the course of prostate cancer progression. D, ANO5 expression is consistently correlated with prostate cancer progression in the The Cancer Genome Atlas cohort. Values between brackets denote the number of patients. Rho: Spearman's rank correlation coefficient. RP, radical prostatectomy
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References

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