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Comparative Study
. 1988 Jun;369(6):425-9.
doi: 10.1515/bchm3.1988.369.1.425.

Molecular cloning of bovine beta-lactoglobulin cDNA

V N Ivanov  1 , E S Judinkova, S I Gorodetsky
Affiliations
Comparative Study

Molecular cloning of bovine beta-lactoglobulin cDNA

V N Ivanov et al. Biol Chem Hoppe Seyler. 1988 Jun.

Abstract

A cDNA library from bovine mammary gland mRNA was constructed in pBR322 and screened by hybrid-selected translation and immunoscreening. Several beta-lactoglobulin clones were identified and sequenced. All clones contained cDNA fragments corresponding to the 3' region of beta-lactoglobulin mRNA. The 3' non-translated region of beta-lactoglobulin mRNA consists of 187 nucleotides; the polyadenylation signal AATAAA occurs 17 nucleotides before the poly(A) tail. The amino-acid sequence predicted from the 3' coding region corresponds completely to the previously determined amino-acid sequence of beta-lactoglobulin.

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