MiR-182 promotes glioma progression by targeting FBXW7

Abstract

Objective: MicroRNAs (miRNAs) are widely considered to play an important role in the tumor progression. In this study, we aimed to investigate the potential biological effects of miR-182 and its target FBXW7 on glioma development.

Methods: Expression data of glioma were procured from TCGA database. Differential analysis was performed to identify the potential differentially expressed miRNA (DEmiRNA), and bioinformatics databases were utilized for target genes prediction. qRT-PCR was conducted to detect miR-182 and western blot was performed to test FBXW7 in protein level. Then the cells were processed for proliferation determination by CCK-8, migration test through wound healing assay, invasion detection via Transwell and apoptosis measurement by flow cytometry. In addition, dual-luciferase reporter gene assay was carried out for evaluation of the targeted relationship between the miR-182 and FBXW7.

Results: MiR-182 was found to be up-regulated in glioma cells while FBXW7 was down-regulated. Besides, miR-182 was predicted to targeted bind with FBXW7 on 3'UTR via bioinformatics methods, and their targeted relationship was further validated by dual-luciferase assay. MiR-182 mimic could significantly promote cell proliferation, migration, invasion and inhibit cell apoptosis, while miR-182 inhibitor had the negative effect. Moreover, FBXW7 knockdown could reverse the inhibitory effect of miR-182 inhibitor on glioma cells.

Conclusion: MiR-182 promotes cell proliferation, migration, invasion and inhibits cell apoptosis in glioma by targeting FBXW7.

Keywords: Apoptosis; FBXW7; Glioma; Migration and invasion; Proliferation; miR-182.