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. 2020 Jan 24:10:1625.
doi: 10.3389/fphar.2019.01625. eCollection 2019.

UPLC-Q-TOF-MS Study of the Mechanism of THSWD for Breast Cancer Treatment

Xianchun Duan  1   2 , Lingyu Pan  1 , Qiuyu Bao  2 , Daiyin Peng  2
Affiliations

UPLC-Q-TOF-MS Study of the Mechanism of THSWD for Breast Cancer Treatment

Xianchun Duan et al. Front Pharmacol. .

Abstract

Taohong Siwu decoction (THSWD) is a classic traditional Chinese medicine (TCM) prescription that is widely used in the clinical treatment of gynecological and cerebrovascular diseases. Here we used a method that coupled ultra-performance liquid chromatography to quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) in which both positive and negative ion modes were established to investigate the major constituents in THSWD. A Waters ACQUITY UPLC BEH C18 column (2.1 mm×100 mm, 1.7 μm) was used to separate the aqueous extract of THSWD. The mobile phase consisted of 0.1% aqueous formic acid (A) and acetonitrile (B). Ninety-five components were identified in two different ion modes, including aromatic acids, flavones, polysaccharides, volatile oils monoterpene glycosides, aromatic cyanogenic glycosides, and others. Pathological changes in tumors and serum expression of interleukin-4 in a mouse model of breast cancer were detected after THSWD treatment. The results showed that THSWD had obvious therapeutic effects. This study establishes a material basis for the use of THSWD in the treatment of breast cancer.

Keywords: Taohong Siwu decoction; UPLC-Q-TOF-MS; breast cancer; components; rats.

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Figures

Figure 1
Figure 1
Base peak chromatograms of THSWD in positive ion mode.
Figure 2
Figure 2
Base peak chromatograms of THSWD in negative ion mode.
Figure 3
Figure 3
Chemical structures of the main constitutions found in THSWD.
Figure 4
Figure 4
Tandem mass spectra and possible fragment pathways of chlorogenic acid in positive ion mode.
Figure 5
Figure 5
Tandem mass spectra and possible fragment pathways of 4-hydroxy-3-butyl-benzoquinone in positive ion mode.
Figure 6
Figure 6
Tandem mass spectra and possible fragment pathways of paeoniflorin in positive ion mode.
Figure 7
Figure 7
Effect of THSWD on tumor cells in mice. Sections were stained with H&E. Magnification, ×200; scale bars, 50 μm. Model group (A), cisplatin group (B), THSWD H group (C), THSWD M group (D), THSWD L group (E).
Figure 8
Figure 8
CD44 expression (×400). Model group (A), cisplatin group (B), THSWD H group (C), THSWD M group (D), THSWD L group (E).
Figure 9
Figure 9
CD117 expression (×400). Model group (A), cisplatin group (B), THSWD H group (C), THSWD M group (D), THSWD L group (E).
Figure 10
Figure 10
CD133 expression (×400). Model group (A), cisplatin group (B), THSWD H group (C), THSWD M group (D), THSWD L group (E).
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