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. 2020 Feb 3:24:5.
doi: 10.1186/s40824-020-0183-9. eCollection 2020.

The efficacy of rhBMP-2 loaded hydrogel composite on bone formation around dental implants in mandible bone defects of minipigs

Hao-Zhen Lyu  1 , Jae Hyup Lee  1   2
Affiliations

The efficacy of rhBMP-2 loaded hydrogel composite on bone formation around dental implants in mandible bone defects of minipigs

Hao-Zhen Lyu et al. Biomater Res. .

Abstract

Background: In dental or orthopedic surgery, bone substitutes are inserted with implants to promote osteogenesis and enhance osseointegration. The purpose of this research was to evaluate the efficacy of rhBMP-2 (recombinant human bone morphogenetic protein-2) loaded hydrogel composite for bone formation around dental implant in minipig mandible bone defect models.

Methods: We made bone defects with a diameter of 4 mm in minipig mandibles and inserted implants of the same size, to mimic the cases of inserting the screws in the bone defect or poor-quality bone. The rhBMP-2 (300 μg) loaded hydrogel composite (0.5 cc) inserted in the bone defect with the implant in the rhBMP-2 group. After 4 weeks, the mandibles were harvested to evaluate the new bone mass around implants using plain radiographs, micro-CT, and histology.

Results: The micro-CT analysis result showed that the quantity of new bone generation around the implant in the rhBMP-2 group was greater than that in the other groups. Comparing the ratios of bone to implant area in three groups by histology, the amount of newly formed bone in the rhBMP-2 group was the most.

Conclusion: The rhBMP-2 loaded hydrogel composite promotes osteogenesis around dental implant in minipig mandible bone defect, and enhance osseointegration between the dental implant and host bone.

Keywords: Bone morphogenetic protein-2; Hydrogel; Minipig; Osseointegration; Osteogenesis.

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Conflict of interest statement

Competing interestsThe authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Radiograph finding. The white arrows indicate the radiolucent shading area around the implants. a The implant group; b The hydrogel group; c The rhBMP-2 group
Fig. 2
Fig. 2
Percent of bone volume (BV/TV) and trabecular number (Tb.N) by micro-CT analysis. a BV/TV: implant vs rhBMP-2, P = 0.055; b Tb.N: implant vs rhBMP-2, P = 0.055
Fig. 3
Fig. 3
Micro-CT finding. The newly formed bone in the rhBMP-2 group is more than that in the other groups. The green arrows indicate new bone that growth into the implant threads. The green triangles indicate the damages of host bone. a The implant group; b The hydrogel group; c The rhBMP-2 group
Fig. 4
Fig. 4
Histological finding. The implant osseointegration in the rhBMP-2 group was the best. Compared to the implant group, the osseointegration of the implant in the hydrogel group was the worst. The green arrows indicate new formed bone around the implant. Hematoxylin and eosin stain(× 40). Scale bar = 1 mm. I: implant; NB: new bone. a The implant group; b The hydrogel group; c The rhBMP-2 group
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