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. 2019 Dec;8(4):181-190.

Genetic diversity analysis and population structure of some Iranian Fenugreek (Trigonella foenum-graecum L.) landraces using SRAP Markers

Marzieh Amiriyan  1 , Abdolali Shojaeiyan  1 , Abbas Yadollahi  1 , Masoud Maleki  1 , Zeinab Bahari  1
Affiliations

Genetic diversity analysis and population structure of some Iranian Fenugreek (Trigonella foenum-graecum L.) landraces using SRAP Markers

Marzieh Amiriyan et al. Mol Biol Res Commun. 2019 Dec.

Abstract

Fenugreek is one of the important edible and medicinal vegetables that have a long history of cultivation and consumption. Characterize the extent of the genetic diversity among landraces will provide a good context for future breeding programs and genetic resource preservation. Genetic diversity and population structure of 88 individuals of eight landraces of Iranian fenugreek evaluated based on SRAP markers. Seventy-two bands generated from 6 primers in which 56 (80.11%) band were polymorph. Hamadan landrace showed the lowest values of percentage of polymorphic loci (67.86), Nei's gene diversity index (0.24), number of effective alleles (1.40) and Shannon's Information index (0.36). Nei's genetic distance matrix revealed the highest genetic distance between Hamadan and Yazd (0.203) and the highest genetic similarity between Mahallat and Varamin (0.036) landraces. The most gene flow was between Mahallat and Varamin landraces (Nm=8.36) and the least was between Shiraz and Hamadan landraces (Nm=0.66). An extent admixture of alleles between the Iranian fenugreek landraces was observed by the population structure. Mantel test indicated that the genetic differentiation and gene flow is not associated with geographic distance in Iranian fenugreek landraces. Our observations indicated SRAP is an efficient technique to reveal genetic diversity and population structure of Iranian fenugreek landrace.

Keywords: Dendrogram; GenAlex; Genetic Diversity; Mantel test; PCOA; Pearson correlation.

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Conflict of interest statement

No conflicts of interest exist.

Figures

Figure 1
Figure 1
Two-dimensional plot from Principal Coordinate Analysis of genetic distance matrix of Iranian fenugreek landraces
Figure 2
Figure 2
Neighbor-Joining dendrogram of SRAP data of Iranian fenugreek landraces
Figure 3
Figure 3
Inference of optimum K based on delta K for SRAP data of 8 Iranian fenugreek landraces
Figure 4
Figure 4
Structure plot of fenugreek landraces based on SRAP data. Each landrace is represented by a single vertical bar, which is partitioned into different colors. Each color represents a genetic cluster and the colored segments shows the individual’s estimated ancestry proportion to each of the genetic clusters.
See this image and copyright information in PMC

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