Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Jan 18;150(2):211-9.
doi: 10.1007/BF00695401.

A suitable method for construction and cloning hybrid plasmids containing EcoRI-fragments of E. coli genome

J I Kozlov, N A Kalinina, L V Gening, B A Rebentish, A Y Strongin, V G Bogush, V G Debabov

A suitable method for construction and cloning hybrid plasmids containing EcoRI-fragments of E. coli genome

J I Kozlov et al. Mol Gen Genet. .

Abstract

A convenient procedure for the isolation of specific EcoRI-fragments of E. coli genome and their amplification on Km-resistance plasmid victor CK delta11 is described. The hybrid molecules were constructued in vitro using EcoRI-digestion, followed by ligation. Then appropriated E. coli strain was transformed with ligated DNA mixture and hybrid plasmids CK delta11-arg+, CK delta11-his+, CK delta11-thr+ and CK delta11-leu+ containing loci of E. coli genome were selected by molecular cloning. The hybrid plasmids obtained consisted of one EcoRI-fragment of initial plasmid CK delta11 and one respective specific portion of E. coli genome.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240-4 - PubMed
    1. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4361-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 - PubMed
    1. Mol Gen Genet. 1973 Jul 31;124(1):1-10 - PubMed
    1. Cell. 1975 Oct;6(2):231-44 - PubMed

LinkOut - more resources