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. 1977 Jan 18;150(2):211-9.
doi: 10.1007/BF00695401.

A suitable method for construction and cloning hybrid plasmids containing EcoRI-fragments of E. coli genome

J I Kozlov, N A Kalinina, L V Gening, B A Rebentish, A Y Strongin, V G Bogush, V G Debabov

A suitable method for construction and cloning hybrid plasmids containing EcoRI-fragments of E. coli genome

J I Kozlov et al. Mol Gen Genet. .

Abstract

A convenient procedure for the isolation of specific EcoRI-fragments of E. coli genome and their amplification on Km-resistance plasmid victor CK delta11 is described. The hybrid molecules were constructued in vitro using EcoRI-digestion, followed by ligation. Then appropriated E. coli strain was transformed with ligated DNA mixture and hybrid plasmids CK delta11-arg+, CK delta11-his+, CK delta11-thr+ and CK delta11-leu+ containing loci of E. coli genome were selected by molecular cloning. The hybrid plasmids obtained consisted of one EcoRI-fragment of initial plasmid CK delta11 and one respective specific portion of E. coli genome.

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References

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