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. 2020 Feb 7;8(2):222.
doi: 10.3390/microorganisms8020222.

Prevalence, Population Diversity and Antimicrobial Resistance of Campylobacter coli Isolated in Italian Swine at Slaughterhouse

Guido Di Donato  1 , Francesca Marotta  2 , Roberta Nuvoloni  3 , Katiuscia Zilli  2 , Diana Neri  2 , Daria Di Sabatino  1 , Paolo Calistri  1 , Elisabetta Di Giannatale  2
Affiliations

Prevalence, Population Diversity and Antimicrobial Resistance of Campylobacter coli Isolated in Italian Swine at Slaughterhouse

Guido Di Donato et al. Microorganisms. .

Abstract

Campylobacter spp. are among the microorganisms most commonly associated with foodborne disease. Swine are known to be the main reservoir of Campylobacter coli and a possible source infection of humans as a result of carcass contamination at slaughter. The aim of this study was to evaluate the prevalence of C. coli contamination in swine carcasses, the antimicrobial resistance (AMR) patterns of isolates and the genetic diversity between strains obtained from swine and those isolated from humans. The prevalence of contamination was higher on carcasses (50.4%) than in faeces (32.9%). The 162 C. coli isolated from swine were examined by pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). The results of PFGE indicated a high genetic diversity among the isolates, with 25 different PFGE types. MLST assigned 51 sequence types (STs) to isolates. The most common genotype was ST-854 (16.04%), ST-9264 (10.49 %) and ST-1016 (6.08 %). Results of AMR showed a high resistance to quinolones and fluoroquinolones together with aminoglycosides and tetracycline. Many strains were multi-resistant with predominant R-type TeSCipNa (57%). Five resistance genes were detected along with mutation in the gyrA gene. A strong correlation between phenotypic and genotypic resistance was found for fluoroquinolone and tetracycline. Genetic profiles obtained in swine isolates were compared to those of 11 human strains. All human strains and 64.19% of animal strains (104/162) were assigned to the ST-828 clonal complex.

Keywords: Campylobacter coli; antimicrobial resistance; molecular analysis.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
In green are the Italian Regions where pigs were bred.
Figure 2
Figure 2
Prevalence and CI in carcasses and faeces during seasons.
Figure 3
Figure 3
Minimum spanning tree generated for 11 human isolates and 162 pig isolates. Each circle represents an allelic profile. The numbers on the connecting lines illustrate the numbers of target genes with differing alleles. The different sources are distinguished by the colors of the circles with blue for human isolates, orange for pig stool and violet for pig carcasses isolates.
Figure 4
Figure 4
Percentage of resistant C. coli isolates from pig and humans. * p ≤ 0.5.
See this image and copyright information in PMC

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