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Comment
. 2020 May 15;201(10):1311-1312.
doi: 10.1164/rccm.202001-0221LE.

Reply to de Steenhuijsen Piters and Bogaert: Bacterial DNA in Fetal Lung Samples May Be Explained by Sample Contamination

Denise Al Alam  1 , Soula Danopoulos  1 , Brendan Grubbs  1 , Nur A'tikah Binte Mohamed Ali  2 , Micheál Mac Aogáin  2 , Sanjay H Chotirmall  2 , David Warburton  1 , Amit Gaggar  3 , Namasivayam Ambalavanan  3 , Charitharth Vivek Lal  3
Affiliations
Comment

Reply to de Steenhuijsen Piters and Bogaert: Bacterial DNA in Fetal Lung Samples May Be Explained by Sample Contamination

Denise Al Alam et al. Am J Respir Crit Care Med. .
No abstract available

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Comment on

  • Human Fetal Lungs Harbor a Microbiome Signature.
    Al Alam D, Danopoulos S, Grubbs B, Ali NABM, MacAogain M, Chotirmall SH, Warburton D, Gaggar A, Ambalavanan N, Lal CV. Al Alam D, et al. Am J Respir Crit Care Med. 2020 Apr 15;201(8):1002-1006. doi: 10.1164/rccm.201911-2127LE. Am J Respir Crit Care Med. 2020. PMID: 31898918 Free PMC article. No abstract available.
  • Bacterial DNA in Fetal Lung Samples May Be Explained by Sample Contamination.
    de Steenhuijsen Piters WAA, Bogaert D. de Steenhuijsen Piters WAA, et al. Am J Respir Crit Care Med. 2020 May 15;201(10):1310-1311. doi: 10.1164/rccm.202001-0050LE. Am J Respir Crit Care Med. 2020. PMID: 32049545 Free PMC article. No abstract available.

References

    1. Al Alam D, Danopoulos S, Grubbs B, Ali NABM, MacAogain M, Chotirmall SH, et al. Human fetal lungs harbor a microbiome signature [letter] Am J Respir Crit Care Med2020;201:1002–1006. - PMC - PubMed
    1. Eisenhofer R, Minich JJ, Marotz C, Cooper A, Knight R, Weyrich LS. Contamination in low microbial biomass microbiome studies: issues and recommendations. Trends Microbiol. 2019;27:105–117. - PubMed
    1. Liu CJ, Liang X, Niu ZY, Jin Q, Zeng XQ, Wang WX, et al. Is the delivery mode a critical factor for the microbial communities in the meconium? EBioMedicine. 2019;49:354–363. - PMC - PubMed
    1. Danopoulos S, Bhattacharya S, Mariani TJ, Al Alam D. Transcriptional characterisation of human lung cells identifies novel mesenchymal lineage markers. Eur Respir J. 2020;55:1900746. - PMC - PubMed
    1. Salter SJ, Cox MJ, Turek EM, Calus ST, Cookson WO, Moffatt MF, et al. Reagent and laboratory contamination can critically impact sequence-based microbiome analyses. BMC Biol. 2014;12:87. - PMC - PubMed