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. 2021 Feb;18(2):492-494.
doi: 10.1038/s41423-020-0371-5. Epub 2020 Feb 13.

Transplantation of murine neonatal cardiac macrophage improves adult cardiac repair

Yandong Li  1 , Haotong Li  1 , Jianqiu Pei  1 , Shengshou Hu  1 , Yu Nie  2
Affiliations

Transplantation of murine neonatal cardiac macrophage improves adult cardiac repair

Yandong Li et al. Cell Mol Immunol. 2021 Feb.
No abstract available

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Implication of neonatal cardiac macrophage ameliorates cardiac repair in myocardial infarction injured adult mice. a Experimental strategy for deleting macrophages using DT in CD11bDTR mice, followed by apical resection at day 1. DT is administrated from day 0 to 2 for consecutive three days. Mice are sacrificed at 21 days after the operation. b IF shows that macrophages infiltration after heart injury is suspended after DT administration in CD11bDTR mice. c Representative images for Masson’s trichrome staining show that heart cannot regenerate but with fibrosis formation in the macrophage-deleted group and DT administration leads to lower quantities of regenerative mice (n=16 for each group). d Representative images of echocardiography in macrophage-deleted mice after apical resection operation. Statistical analysis shows the lower EF% and FS% in macrophage-deleted mice. Data are mean ± SEM. ****P<0.0001. e IF shows that macrophages deletion suspends cardiomyocytes proliferation identified by decreased pH3 positive α-actinin. f Scheme of deletion of macrophages in adult mice, transplantation of neonatal cardiac macrophages into adult injured heart as a treatment therapy. Strategy of injecting GFP positive macrophages isolated from Cx3cr1GFP/+mice to CD11bDTR adult mice following myocardial infarction operation. g Kaplan-Meier survival curve in adult mice following myocardial injection induction shows that macrophages transplantation has the highest survival rate. Data are mean ± SEM. *P<0.05, **P<0.01. h Representative images for Masson’s trichrome staining shows macrophages injection has the smallest infarcted area in adult CD11bDTR mice (PBS+DMEM, n=12; DT+DMEM, n=14; DT+Macrophage n=14). i Neonatal cardiac macrophages (GFP positive) can be detected in adult mouse heart after injection. j Representative images of echocardiography in macrophage-injected mice after myocardial infarction operation. Statistical analysis shows the highest EF% and FS% in macrophage-injected adult mice. Data are mean±SEM. *P<0.05. k If shows that macrophages transplantation can induced adult cardiomyocytes proliferation (n=6 for each group). Data are mean±SEM. *P<0.05, **P<0.01
See this image and copyright information in PMC

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