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. 2020 Feb 4;6(2):e03316.
doi: 10.1016/j.heliyon.2020.e03316. eCollection 2020 Feb.

Comparative evaluation of urinary dipstick and pH-meter for cattle urine pH measurement

Ali Afsahi  1 , Mahmood Ahmadi-Hamedani  2 , Mohammad Khodadi  1
Affiliations

Comparative evaluation of urinary dipstick and pH-meter for cattle urine pH measurement

Ali Afsahi et al. Heliyon. .

Abstract

Urinary dipsticks are often used for the diagnosis of kidney, liver, metabolic, and urogenital diseases in cattle clinical practice for the simplicity of use and ease of access. The aims of the present study were to evaluate the agreement between urinary dipsticks and pH-meter for the urine pH in dairy cattle, and also to compare the urine pH before and after centrifugation from each method. The agreement between urine dipstick and pH-meter methods before and after centrifuge were calculated by Passing-Bablok regression analysis demonstrated constant differences (intercept) at 0.60 and -1.01 and positive proportional differences (slope) at 0.94 and 1.13; respectively. Total bias estimated by Bland-Altman plot analysis before (0.20) and after (0.14) centrifuge were lower than the acceptable bias in urine samples. The regression analysis of this study emphasized that the urinary dipstick can be used to determine the cattle urine.

Keywords: Agriculture; Bland-altman; Cattle; Health sciences; Passing-bablock; Urine; Veterinary medicine.

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Figures

Figure 1
Figure 1
A. Passing and Bablok regression plots between the pH measurement by dipstick (Cattle Dipstick Before centrifugation—CStickB) vs the pH-meter (Cattle pH-meter Before centrifugation—CpH-meterB) in cattle urine. This graph shows the observations with the regression line (solid line), the confidence interval for the regression line (dashed lines). B. Bland–Altman plots showing the difference between the pH measured by the pH-meter (Cattle pH-meter Before centrifugation —CpHmeterB) vs dipstick (Cattle Dipstick Before centrifugation—CStickB), against the mean of the 2 methods in cattle urine.
Figure 2
Figure 2
A. Passing and Bablok regression plot between the values measured by the pH-meter Before (CpHmeterB) and After centrifugation (CpHmeterA) in cattle. B. Bland–Altman plot showing the difference between pH measurements by the pH-meter Before and After centrifugation (CpHmeterB–CpHmeterA), against the mean of the 2 measurements (mean of CpHmeterA and CpHmeterB) in cattle urine.
Figure 3
Figure 3
Bland–Altman plots showing the difference between the pH measured by the pH-meter (Cattle pH-meter After centrifugation —CpHmeterA) vs dipstick (Cattle Dipstick After centrifugation—CStickA), against the mean of the 2 methods in cattle urine.
See this image and copyright information in PMC

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