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. 2020 Nov 5;71(8):e215-e217.
doi: 10.1093/cid/ciaa145.

Transmission of NS5A-Inhibitor Resistance-Associated Substitutions Among Men Who Have Sex With Men Recently Infected with Hepatitis C Virus Genotype 1a

Stephanie Popping  1 , Rosanne Verwijs  2 , Lize Cuypers  3   4 , Mark A Claassen  5 , Guido E van den Berk  6 , Anja De Weggheleire  7 , Joop E Arends  8 , Anne Boerekamps  2 , Richard Molenkamp  1 , Marion P Koopmans  1 , Annelies Verbon  2 , Charles A B Boucher  1 , Bart J Rijnders  2 , David A M C van de Vijver  1 ; Dutch Acute HCV in HIV Study Investigators
Collaborators, Affiliations

Transmission of NS5A-Inhibitor Resistance-Associated Substitutions Among Men Who Have Sex With Men Recently Infected with Hepatitis C Virus Genotype 1a

Stephanie Popping et al. Clin Infect Dis. .

Abstract

The transmission of direct-acting antiviral resistance-associated substitutions (RAS) could hamper hepatitis C virus (HCV) cure rates and elimination efforts. A phylogenetic analysis of 87 men who have sex with men recently infected with HCV genotype 1a placed one-third (28/87) in a large cluster, in which 96% harbored NS5A M28V RAS.

Keywords: HIV co-infected MSM; hepatitis C virus; phylogenetic analysis; resistant associated substitutions; the WHO 2030 elimination goals.

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Figures

Figure 1.
Figure 1.
Phylogenetic analysis of a concatenated NS5A and NS5B alignment showed clusters including 28 MSM (n = 27 living with HIV; n = 1 living without HIV). Of the individuals in this cluster, 96% harbored an NS5A M28V–containing variant. All patients in this cluster treated with an NS5A-inhibitor (grazoprevir and elbasvir for 8 weeks) obtained a sustained virologic response. The sequences depicted with green were collected prior to the massive direct-acting antiviral therapy uptake in 2015, and the sequences depicted with blue were collected in the years thereafter. The cluster had a bootstrap value and Shimodaira Hasegawaa–like approximate likelihood ratio of >90 and a genetic distance threshold of <3%. For methods, see Supplementary Materials. Abbreviations: HIV, human immunodeficiency virus; MSM, men who have sex with men; RAS, resistance-associated substitutions.
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