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. 2020 Mar;55(2):163-171.
doi: 10.1002/lipd.12220. Epub 2020 Feb 13.

Adipogenic and Myogenic Potentials of Chicken Embryonic Fibroblasts in vitro: Combination of Fatty Acids and Insulin Induces Adipogenesis

Dong-Hwan Kim  1 , Joonbum Lee  1   2 , Yeunsoo Suh  1 , Michael Cressman  1 , Sang Suk Lee  3 , Kichoon Lee  1   2
Affiliations

Adipogenic and Myogenic Potentials of Chicken Embryonic Fibroblasts in vitro: Combination of Fatty Acids and Insulin Induces Adipogenesis

Dong-Hwan Kim et al. Lipids. 2020 Mar.

Abstract

The regulation of adipocyte differentiation is an important factor for production efficiency and meat quality in the poultry industry. The purpose of this study was to develop a new in vitro model of adipogenic differentiation of chicken embryonic fibroblasts (CEF). In this study, CEF were isolated at embryonic day (E) 5, and adipogenic differentiation was induced with supplementation of fatty acids (FA) and/or insulin (Ins) for 48 h. Oil-Red-O staining showed that lipid accumulation in E5 CEF was greater when supplemented with a combination of FA and Ins (FI) than other treatment groups (p < 0.05). Genes involved in differentiation of preadipocytes, fatty acid transport, and triacylglycerol synthesis were upregulated in the FI group compared to all other treatment groups (p < 0.01). Under myogenic media, the E5 CEF formed myotubes and expressed myogenic markers, myosin heavy chain (MHC), and myogenin (MyoG), suggesting myogenic potential of E5 CEF. To determine the permissive age window for adipogenic differentiation of CEF, E5, E6, and E7 CEF were induced for adipogenesis with FI treatment in 1%, 5%, or 10% chicken serum (CS). Among all embryonic ages, E5 with 10% CS showed the most lipid accumulation and the least myotube formation with the lowest expression of MHC and MyoG. These data indicate both adipogenic and myogenic potentials of E5 CEF, providing a new in vitro model for a better understanding of the processes of adipogenic and myogenic differentiation in chickens.

Keywords: Adipogenesis; Chicken embryonic fibroblast; Fatty acid; Insulin; Myogenesis.

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References

    1. Ahn, J., Kim, D. H., Suh, Y., Lee, J. W., & Lee, K. (2018) Adipose-specific expression of mouse Rbp7 gene and its developmental and metabolic changes. Gene, 670:38-45.
    1. Chen, P., Suh, Y., Choi, Y. M., Shin, S., & Lee, K. (2014) Developmental regulation of adipose tissue growth through hyperplasia and hypertrophy in the embryonic Leghorn and broiler. Poultry Science, 93:1809-1817.
    1. Choi, Y. M., Suh, Y., Shin, S., & Lee, K. (2014) Skeletal muscle characterization of Japanese quail line selectively bred for lower body weight as an avian model of delayed muscle growth with hypoplasia. PLoS One, 9:e95932.
    1. Green, H., & Meuth, M. (1974) An established pre-adipose cell line and its differentiation in culture. Cell, 3:127-133.
    1. Gupta, R. K., Arany, Z., Seale, P., Mepani, R. J., Ye, L., Conroe, H. M., … Spiegelman, B. M. (2010) Transcriptional control of preadipocyte determination by Zfp423. Nature, 464:619-623.

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