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. 2020 Feb 12;21(4):1227.
doi: 10.3390/ijms21041227.

Probiotic Properties and Neuroprotective Effects of Lactobacillus buchneri KU200793 Isolated from Korean Fermented Foods

Min-Jeong Cheon  1 , Sung-Min Lim  1 , Na-Kyoung Lee  1 , Hyun-Dong Paik  1
Affiliations

Probiotic Properties and Neuroprotective Effects of Lactobacillus buchneri KU200793 Isolated from Korean Fermented Foods

Min-Jeong Cheon et al. Int J Mol Sci. .

Abstract

The purpose of this study was to evaluate the probiotic characteristics and neuroprotective effects of bacteria isolated from Korean fermented foods. Three bacterial strains (Lactobacillus fermentum KU200060, Lactobacillus delbrueckii KU200171, and Lactobacillus buchneri KU200793) showed potential probiotic properties, such as high tolerance against artificial gastric juice and bile salts, sensitivity to antibiotics, nonproduction of carcinogenic enzymes, and high adhesion to intestinal cells. Heat-killed L. fermentum KU200060 and L. buchneri KU200793 showed higher antioxidant activity than heat-killed L. delbrueckii KU200171. The conditioned medium (CM) was used to evaluate the reaction between HT-29 cells and each heat-killed strain. All CMs protected SH-SY5Y cells from 1-methyl-4-phenylpyridinium (MPP+)-induced toxicity. The expression of brain-derived neurotropic factor (BDNF) mRNA in HT-29 cells treated with CM containing heat-killed L. buchneri KU200793 was the highest. The CM significantly reduced the Bax/Bcl-2 ratio and increased BDNF mRNA expression in SH-SY5Y cells treated with MPP+. These results indicate that L. buchneri KU200793 can be used as a prophylactic functional food, having probiotic potential and neuroprotective effects.

Keywords: Lactobacillus buchneri; gut-brain-axis; kimchi; neuroprotective effect; probiotics.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Neuroprotective effect of conditioned medium (CM) on MPP+ (1 mM)-stressed SH-SY5Y cells. L. rhamnosus GG, CM of heat-killed L. rhamnosus GG; L. fermentum 200060, CM of heat-killed L. fermentum KU200060; L. delbrueckii 200171, CM of heat-killed L. delbrueckii KU200171; and L. buchneri 200793, CM of heat-killed L. buchneri KU200793. All values are expressed as mean ± standard error of triplicate experiments. Different superscript letters on each bar represent significant differences (p < 0.05).
Figure 2
Figure 2
mRNA expression levels of the brain-derived neurotropic factor (BDNF) gene in heat-killed lactic acid bacteria (LAB) treated HT-29 cells using RT-PCR. The fold change was calculated based on normalization with GAPDH gene expression. Control group was treated with PBS. L. rhamnosus GG, heat-killed L. rhamnosus GG; L. fermentum 200060, heat-killed L. fermentum KU200060; L. delbrueckii 200171, heat-killed L. delbrueckii KU200171; and L. buchneri 200793, heat-killed L. buchneri KU200793. All values are expressed as mean ± standard error of triplicate experiments. Different superscript letters on each bar represent significant differences (p < 0.05).
Figure 3
Figure 3
mRNA expression of (A) BDNF and (B) apoptosis-related genes (Bax/Bcl-2 ratio) in SH-SY5Y cells using RT-PCR. L. rhamnosus GG, CM of heat-killed L. rhamnosus GG; L. fermentum 200060, CM of heat-killed L. fermentum KU200060; L. delbrueckii 200171, CM of heat-killed L. delbrueckii KU200171; and L. buchneri 200793, CM of heat-killed L. buchneri KU200793. All values are mean ± standard error of triplicate experiments. Different superscript letters on each bar represent significant differences (p < 0.05).
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References

    1. Mary E.S. Probiotics: Considerations for human health. Nutr. Rev. 2003;61:91–99. - PMC - PubMed
    1. Yang S.J., Lee J.E., Lim S.M., Kim Y.J., Lee N.K., Paik H.D. Antioxidant and immune-enhancing effects of probiotic Lactobacillus plantarum 200655 isolated from kimchi. Food Sci. Biotechnol. 2019;28:491–499. doi: 10.1007/s10068-018-0473-3. - DOI - PMC - PubMed
    1. Dinan T.G., Stanton C., Cryan J.F. Psychobiotics: A Novel Class of Psychotropic. Biol. Psychiatry. 2013;74:720–726. doi: 10.1016/j.biopsych.2013.05.001. - DOI - PubMed
    1. Perez-Pardo P., Kliest T., Dodiya H.B., Broersen L.M., Garssen J., Keshavarzian A., Kraneveld A.D. The gut-brain axis in Parkinson’s disease: Possibilities for food-based therapies. Eur. J. Pharmacol. 2017;817:86–95. doi: 10.1016/j.ejphar.2017.05.042. - DOI - PubMed
    1. Warda A.K., Rea K., Fitzgerald P., Hueston C., Gonzalez-Tortuero E., Dinan T.G., Hill C. Heat-killed lactobacilli alter both microbiota composition and behavior. Behav. Brain Res. 2019;362:213–223. doi: 10.1016/j.bbr.2018.12.047. - DOI - PubMed

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