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. 2020 Feb 12;21(4):1233.
doi: 10.3390/ijms21041233.

The Effect of Silver Nanoparticles on Antioxidant/Pro-Oxidant Balance in a Murine Model

Anca Oana Docea  1 , Daniela Calina  2 , Ana Maria Buga  3 , Ovidiu Zlatian  4 , M M B Paoliello  5   6 , George Dan Mogosanu  7 , Costin Teodor Streba  8 , Elena Leocadia Popescu  9 , Alexandra Elena Stoica  10 , Alexandra Catalina Bîrcă  10 , Bogdan Ștefan Vasile  10 , Alexandru Mihai Grumezescu  10 , Laurentiu Mogoanta  11
Affiliations

The Effect of Silver Nanoparticles on Antioxidant/Pro-Oxidant Balance in a Murine Model

Anca Oana Docea et al. Int J Mol Sci. .

Abstract

This study aimed to evaluate the subacute effect of two types of Ag-NPs(EG-AgNPs and PVP-EG-AgNPs) on antioxidant/pro-oxidant balance in rats. Seventy Wistar rats (35 males and 35 females) were divided in 7 groups and intraperitoneally exposed for 28 days to 0, 1, 2 and 4 mg/kg bw/day EG-Ag-NPs and 1, 2 and 4 mg/kg bw/day PVP- EG-Ag-NPs. After 28 days, the blood was collected, and the total antioxidant capacity (TAC), thiobarbituric reactive species (TBARS),protein carbonyl (PROTC) levels, reduced glutathione (GSH) levels and catalase (CAT) activity were determined. EG-Ag-NPs determined protective antioxidant effects in a dose-dependent manner. The exposure to the 4 mg/kg bw/day EG-Ag-NPs determines both in males and females a significant increase in TAC and CAT and a significant decrease in TBARS and PROTC only in females. The PVP-EG-AgNPs showed a different trend compared to EG-AgNPs. At 4 mg/kg bw/day the PVP-EG-AgNPs induce increased PROTC levels and decreased GSH (males and females) and TAC levels (males). The different mechanisms of EG-AgNPs and PVP-EG-AgNPs on antioxidant-/pro-oxidant balance can be explained by the influence of coating agent used for the preparation of the nanoparticles in the formation and composition of protein corona that influence their pathophysiology in the organism.

Keywords: antioxidant activity; oxidative stress; silver nanoparticles; subacute toxicity.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
images recorded for (a) EG-AgNPs and (b) PVP-EG-AgNPs.
Figure 2
Figure 2
Histogram indicating the particle size distribution: (a)Ag-NPs functionalized with ethylene glycol (EG-Ag NPs); (b)Ag-NPs functionalized with polyvinylpyrolidone and ethylene glycol.
Figure 3
Figure 3
Representation of EG-Ag-NPs synthesis.
Figure 4
Figure 4
Design of the study.
See this image and copyright information in PMC

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