Effects of high light and temperature on Microcystis aeruginosa cell growth and β-cyclocitral emission

Abstract

Cyanobacteria always massively grow and even occur blooms in summer, with releasing amount of β-cyclocitral. To uncover the effects of summer high irradiance and temperature on cyanobacterial growth and β-cyclocitral emission, the cell growth, reactive oxygen species (ROS) levels, photosynthetic pigment content, chlorophyll fluorescence and β-cyclocitral emission were investigated in Microcystis aeruginosa under high light and temperature. Compared to the control under 50 μmol m^-2·s^-1, the cell growth was promoted under 100 μmol m^-2·s^-1, but inhibited under 500 and 1000 μmol m^-2·s^-1. The inhibition was also detected under high temperature at 30 and 35 °C in contrast to the control at 25 °C. Under high light and high temperature, M. aeruginosa increased ROS levels and reduced photosynthetic pigment content and photosystem II (PSII) efficiency, which resulted in the inhibition on cell growth. With increasing the light intensity and temperature, ¹O₂ levels gradually increased, while β-carotene content gradually decreased by quenching ¹O₂, with increasing β-cyclocitral emission. In summer, high irradiance and temperature not benefited the growth of cyanobacteria, but the emission of β-cyclocitral derived from β-carotene quenching ¹O₂ may offset the disadvantages by poisoning other algae.

Keywords: Cyanobacteria; Environmental stressors; High light; High temperature; β-Cyclocitral.