Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Mar;17(3):279-282.
doi: 10.1038/s41592-020-0746-7. Epub 2020 Feb 17.

Chemogenetic Control of Nanobodies

Helen Farrants  1   2 , Miroslaw Tarnawski  3 , Thorsten G Müller  4 , Shotaro Otsuka  5   6 , Julien Hiblot  1 , Birgit Koch  1 , Moritz Kueblbeck  5 , Hans-Georg Kräusslich  4 , Jan Ellenberg  5 , Kai Johnsson  7   8
Affiliations

Chemogenetic Control of Nanobodies

Helen Farrants et al. Nat Methods. 2020 Mar.

Abstract

We introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switched on and off with small molecules. By controlling the cellular localization of GFP fusion proteins, the engineered nanobody allows interrogation of their roles in basic biological processes, an approach that should be applicable to numerous previously described GFP fusions. We also outline how the binding affinities of other nanobodies can be controlled by small molecules.

PubMed Disclaimer

Comment in

References

    1. Hamers-Casterman, C. et al. Naturally occurring antibodies devoid of light chains. Nature 363, 446–448 (1993). - DOI
    1. Muyldermans, S. Nanobodies: natural single-domain antibodies. Annu. Rev. Biochem. 82, 775–797 (2013). - DOI
    1. Ingram, J. R., Schmidt, F. I. & Ploegh, H. L. Exploiting nanobodies’ singular traits. Annu. Rev. Immunol. 36, 695–715 (2018). - DOI
    1. Stein, V. & Alexandrov, K. Synthetic protein switches: design principles and applications. Trends Biotechnol. 33, 101–110 (2015). - DOI
    1. Oakes, B. L. et al. Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch. Nat. Biotechnol. 34, 646–651 (2016). - DOI - PubMed - PMC

Publication types

MeSH terms