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. 2020 Feb 13;5(1):25.
doi: 10.3390/tropicalmed5010025.

First Draft Genome of the Trypanosomatid Herpetomonas muscarum ingenoplastis through MinION Oxford Nanopore Technology and Illumina Sequencing

Claudia M d'Avila-Levy  1   2 , Bertrand Bearzatto  3 , Jérôme Ambroise  3 , Raphaël Helaers  2 , Anzhelika Butenko  4   5 , Vyacheslav Yurchenko  5   6 , Karina A Morelli  1   7 , Helena L C Santos  1 , Pascal Brouillard  2 , Philippe Grellier  8 , Jean-Luc Gala  3 , Miikka Vikkula  2
Affiliations

First Draft Genome of the Trypanosomatid Herpetomonas muscarum ingenoplastis through MinION Oxford Nanopore Technology and Illumina Sequencing

Claudia M d'Avila-Levy et al. Trop Med Infect Dis. .

Abstract

Here, we present first draft genome sequence of the trypanosomatid Herpetomonas muscarum ingenoplastis. This parasite was isolated repeatedly in the black blowfly, Phormia regina, and it forms a phylogenetically distinct clade in the Trypanosomatidae family.

Keywords: Trypanosomatidae; genome assembly; insect trypanosomatids; monoxenous trypanosomatids; whole genome.

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Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

Figures

Figure 1
Figure 1
Phylogenetic analysis by ML and BI of H. muscarum ingenoplastis. The tree is based on the partial sequences of gGAPDH from COLPROT021 and GenBank sequences. The numbers at the top of each node denote Bayesian posterior probability and maximum likelihood bootstrap values. Dashes (-) indicate bootstrap support below 70% or different topology. The tree was rooted with the sequences from Paratrypanosoma confusum. Double-crossed branches are at 50% of their original lengths. The scale bar denotes the number of substitutions per site.
See this image and copyright information in PMC

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