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. 2020 Mar 27;126(7):923-925.
doi: 10.1161/CIRCRESAHA.119.315653. Epub 2020 Feb 19.

RNA Sequencing Analysis of Induced Pluripotent Stem Cell-Derived Cardiomyocytes From Congenital Heart Disease Patients

Tomoya Kitani #  1   2 , Lei Tian #  1 , Tiejun Zhang #  1 , Ilanit Itzhaki  1 , Joe Z Zhang  1 , Ning Ma  1 , Chun Liu  1 , June-Wha Rhee  1   3 , Anitra W Romfh  3 , George K Lui  3 , Joseph C Wu  1   3
Affiliations

RNA Sequencing Analysis of Induced Pluripotent Stem Cell-Derived Cardiomyocytes From Congenital Heart Disease Patients

Tomoya Kitani et al. Circ Res. .
No abstract available

Keywords: genomics; heart defects, congenital; induced pluripotent stem cells; sequence analysis, RNA; stem cells.

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Figures

Figure 1:
Figure 1:. Transcriptomic analysis of iPSC-CMs from patients with congenital heart disease.
(A) Schematic showing generation of iPSC-CMs and the experimental workflow. HLHS: hypoplastic left heart syndrome; TA: tricuspid atresia; DORV: double-outlet right ventricle; DILV: double inlet left ventricle. (B) PCA of gene expression levels in 5 non-CHD-iPSC-CMs (black), 5 SVD-iPSC-CMs (orange), and 5 TOF-iPSC-CMs (green). (C) Heatmap plot of all DEGs across all samples. (D) GO biological process analysis of DEGs in SVD-iPSC-CMs and TOF-iPSC-CMs. (E) Regulatory gene network in SVD-iPSC-CMs showing the relationship between the enriched TF (circle in pink) and their target genes (small nodes in light blue). (F) Venn diagram depicting the number of up-regulated and down-regulated genes in TOF-iPSC-CMs and TOF-heart tissue.
See this image and copyright information in PMC

References

    1. Bouma BJ, Mulder BJ. Changing landscape of congenital heart disease. Circ Res. 2017;120:908–922. - PubMed
    1. Burridge PW, Matsa E, Shukla P, Lin ZC, Churko JM, Ebert AD, Lan F, Diecke S, Huber B, Mordwinkin NM, et al. Chemically defined generation of human cardiomyocytes. Nat Methods. 2014;11:855–860. - PMC - PubMed

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