Published Erratum
doi: 10.1073/pnas.2001373117.
Epub 2020 Feb 18.
Correction for Heng et al., BBX4, a phyB-interacting and modulated regulator, directly interacts with PIF3 to fine tune red light-mediated photomorphogenesis
- PMID: 32071210
- PMCID: PMC7049156
- DOI: 10.1073/pnas.2001373117
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Published Erratum
Correction for Heng et al., BBX4, a phyB-interacting and modulated regulator, directly interacts with PIF3 to fine tune red light-mediated photomorphogenesis
Proc Natl Acad Sci U S A.
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No abstract available
Figures
phyB genetically and physically interacts with BBX4. (A and B) Hypocotyl phenotype (A) and length (B) of 4-d-old Col, bbx4-1, phyB-9, and bbx4-1 phyB-9 seedlings grown in R (115.8 μmol/m2/s) light. The unit of hypocotyl length is millimeters. The experiments were performed 3 times with similar results. The graphs depict one of these experiments. Error bars represent SE (n ≥ 20). Letters above the bars indicate significant differences (P < 0.05), as determined by 1-way ANOVA with Tukey’s post hoc analysis. (C and D) Hypocotyl phenotype (C) and length (D) of 4-d-old Col, YFP-BBX4 #6, phyB-9, and YFP-BBX4 phyB-9 #6 seedlings grown in R light (115.8 μmol/m2/s). The unit of hypocotyl length is millimeters. The experiments were performed 3 times, with similar results. The graphs depict one of these experiments. Error bars represent SE (n ≥ 20). Letters above the bars indicate significant differences (P < 0.05), as determined by 1-way ANOVA with Tukey’s post hoc analysis. (E) Yeast two-hybrid interactions between the BBX4 and phyB. (F) Semi-in vivo pull-down assay of BBX4 with phyB. Total plant protein was extracted from 4-d-old phyB-myc transgenic seedlings grown in R light (115.8 μmol/m2/s). Equal amounts of MBP and MBP-BBX4 proteins were added to total plant protein extracts. The asterisk indicates MBP-BBX4. Actin served as a negative control. (G) BBX4 and phyB colocalize to the nuclear bodies in tobacco cells. CFP-BBX4 and YFP-phyB were transiently coexpressed in tobacco leaves. CFP-GST and YFP-GST served as negative controls. (Scale bars: 5 µm.) (H) BiFC assay showing the interaction of BBX4 with phyB in R light. BBX4 and phyB were fused to the N- and C-terminal fragments of YFP (YFPN and YFPC, respectively). Unfused YFPN and YFPC fragments served as negative controls. (Scale bars: 20 μm.)
BBX4 genetically and physically interacts with PIF3. (A and B) Hypocotyl phenotype (A) and length (B) of 4-d-old Col, bbx4-1, pif3-1 and bbx4-1 pif3-1 seedlings grown in R light (115.8 μmol/m2/s). The unit of hypocotyl length is millimeters. The experiments were performed 3 times, with similar results. The graphs depict one of these experiments. Error bars represent SE (n ≥ 20). Letters above the bars indicate significant differences (P < 0.05), as determined by 1-way ANOVA with Tukey’s post hoc analysis. (C) Yeast two-hybrid interactions between the BBX4 and PIF3. (D) FRET between CFP-PIF3 and YFP-BBX4 analyzed by acceptor bleaching in nuclei. (Top) Representative prebleach nuclei coexpressing YFP-BBX4 and CFP-PIF3 excited with a 514-nm or 405-nm laser, resulting in emission from YFP or CFP, respectively. (Bottom) The same nuclei after bleaching excited with a 514-nm or 405-nm laser. (E) The relative intensities of both YFP and CFP inside the nuclei were measured once before and twice after the bleaching, as indicated in D. (F) BiFC assay showing the interaction of BBX4 with PIF3 in red light. BBX4 and PIF3 were fused to the N- and C-terminal fragments of YFP (YFPN and YFPC, respectively). Unfused YFPN and YFPC fragments served as negative controls. (Scale bars: 40 μm.)
Erratum for
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BBX4, a phyB-interacting and modulated regulator, directly interacts with PIF3 to fine tune red light-mediated photomorphogenesis.Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):26049-26056. doi: 10.1073/pnas.1915149116. Epub 2019 Nov 27. Proc Natl Acad Sci U S A. 2019. PMID: 31776262 Free PMC article.
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