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Editorial
. 2020 Mar;30(3):189-190.
doi: 10.1038/s41422-020-0290-0.

Virus against virus: a potential treatment for 2019-nCov (SARS-CoV-2) and other RNA viruses

Tuan M Nguyen #  1 , Yang Zhang #  1 , Pier Paolo Pandolfi  2   3
Affiliations
Editorial

Virus against virus: a potential treatment for 2019-nCov (SARS-CoV-2) and other RNA viruses

Tuan M Nguyen et al. Cell Res. 2020 Mar.
No abstract available

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1. Analysis of genomic variations between 2019-nCov(SARS-CoV-2) viruses from infected patients in different countries and a flexible CRISPR/Cas13d strategy for treating 2019-nCov(SARS-CoV-2) virus infection and countering its evolution.
a, b Sequence analysis of 2019-nCov(SARS-CoV-2) virus RNA genome with available complete sequences from 19 infected patients in China, USA and Australia. Lineage tree (a) and peptide sequence alignment (b) for a portion of the polypeptide ORF8 of 2019-nCov (SARS-CoV-2), showing sequence variation between the 2019-nCov (SARS-CoV-2) viruses from different patients. The sequences were extracted from GenBank then aligned with MUSCLE algorithm and visualized with Jalview. Red arrows in (b) indicate regions with variants. Geographical locations and GenBank IDs of the 19 patients are shown. c A model for Cas13d cleavage of 2019-nCov (SARS-CoV-2) RNA genome. d Number of guide RNAs that can be designed to cleave each peptide-encoding region of 2019-nCov (SARS-CoV-2) RNA genome without affecting the human genome. All possbile guide RNAs (gRNAs) containing 22 nt spacer sequences were generated for peptide-encoding regions of 2019-nCov (SARS-CoV-2) RNA genome then mapped to human genome with Bowtie. Guide RNAs with no alignment to human genome allowing up to 2 mismatches were considered to be specific to the 2019-nCov (SARS-CoV-2) RNA genome without human genome recognition. e Schematic for AAV design carrying Cas13d effector and a three-gRNA array for treatment of patients with 2019-nCov (SARS-CoV-2) infection. ITR inverted terminal repeats.
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References

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