Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2020 Jan;12(1):5-11.
doi: 10.15171/mejdd.2020.157.

Signature of Gut Microbiome by Conventional and Advanced Analysis Techniques: Advantages and Disadvantages

Sama Rezasoltani  1 , Dorrieh Ahmadi Bashirzadeh  1 , Ehsan Nazemalhosseini Mojarad  2 , Hamid Asadzadeh Aghdaei  2 , Mohsen Norouzinia  1 , Shabnam Shahrokh  1
Affiliations
Review

Signature of Gut Microbiome by Conventional and Advanced Analysis Techniques: Advantages and Disadvantages

Sama Rezasoltani et al. Middle East J Dig Dis. 2020 Jan.

Abstract

Gut microbiota is considered as a human organ with its own specific functions and complexity. Development of novel techniques such as shut gun sequencing, metagenomics, and next-generation sequencing (NGS) has enabled bypassing the traditional culturedependent bias and has significantly expanded our understanding of the composition, diversity, and roles of the gut microbiota in human health and diseases. Although amplicon sequencing characterizes the taxonomic composition of the gut microbiome, it is impossible to cover the direct evidence of the microbial biological functions related to the gut microbial community. Hence, the critical next step for gut microbiome studies is shifting from gene/ genome-centric analysis to mechanism-centric techniques by integrating omics data with experimental results. Realizing gut microbial diversity and their bioactive metabolites function will provide insight into the clinical application of gut microbiota in diagnoses and treatments of several diseases. In this review, we focused on explaining the conventional and advanced microbiome analysis techniques regarding gut microbiota investigation with considering the advantages and disadvantages of the platforms.

Keywords: Advantages; Disadvantages; Gut microbiota; Microbiome analysis techniques.

PubMed Disclaimer

Conflict of interest statement

CONFLICT OF INTEREST The authors declare no conflict of interest related to this work.

References

    1. Cherng-Shyang Ch, Cheng-Yuan K. Current understanding of the gut microbiota shaping mechanisms. J Biomed Sci. 2019;26:59. - PMC - PubMed
    1. Rezasoltani S, Dabiri H, Asadzadeh-Aghdaei H, Sepahi AA, Modarressi MH, Nazemalhosseini-Mojarad E. The gut microflora assay in patients with colorectal cancer: in feces or tissue samples? Iran J Microbiol. 2019;11:1–6. doi: 10.18502/ijm.v11i1.696. - DOI - PMC - PubMed
    1. Maukonen J, Characterization of the human predominant fecal microbiota-With special focus on the Clostridial clusters IV and XIVa, Dissertation. Espoo 2012; VTT Technical Research Centre of Finland.
    1. Rezasoltani S, Asadzadeh Aghdaei H, Dabiri H, Akhavan Sepahi A, Modarressi MH, Nazemalhosseini Mojarad E. The association between fecal microbiota and different types of colorectal polyp as precursors of colorectal cancer. Microb Pathog. 2018;124:244–249. doi: 10.1016/j.micpath.2018.08.035. - DOI - PubMed
    1. Rezasoltani S, Sharafkhah M, Asadzadeh Aghdaei H, Nazemalhosseini Mojarad E, Dabiri H, Akhavan Sepahi A. et al. Applying simple linear combination, multiple logistic and factor analysis methods for candidate fecal bacteria as novel biomarkers for early detection of adenomatous polyps and colon cancer, 2018. J Microbiol Methods. 2018;155:82–88. doi: 10.1016/j.mimet.2018.11.007. - DOI - PubMed

LinkOut - more resources