Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Dec 27:2019:8406036.
doi: 10.1155/2019/8406036. eCollection 2019.

LEA Gene Expression Assessment in Advanced Mutant Rice Genotypes under Drought Stress

Zarifth Shafika Kamarudin  1 , Mohd Rafii Yusop  1   2 , Mohd Razi Ismail  2 , Mahmud Tengku Muda Mohamed  1 , Abdul Rahim Harun  3 , Oladosu Yusuff  2 , Usman Magaji  2 , Arolu Fatai  2
Affiliations

LEA Gene Expression Assessment in Advanced Mutant Rice Genotypes under Drought Stress

Zarifth Shafika Kamarudin et al. Int J Genomics. .

Abstract

Late embryogenesis abundant (LEA) proteins are primarily found in plants stem, roots, and other organs and play significant roles in tolerance to several abiotic stresses. Plants synthesize a discrete set of LEA proteins in response to drought stress. In this study, the expression patterns of LEA genes were investigated in two advanced mutant rice genotypes subjected to the drought stress condition and different physiological traits including photosynthetic rate, leaf chlorophyll content, and photosystem II (PSII) photochemical efficiency (Fv/Fm) which were analyzed to confirm their drought tolerance. Five LEA genes (OsLEA1, OsLEA2, OsLEA3, OsLEA4, and OsLEA5) were used in the evaluation of rice genotypes and were significantly upregulated by more than 4-fold for MR219-4 and MR219-9. The upregulated genes by these two varieties showed high similarity with the drought-tolerant check variety, Aeron1. This indicates that these advanced mutant genotypes have better tolerance to drought stress. The changes in the expression level of LEA genes among the selected rice genotypes under drought stress were further confirmed. Hence, LEA genes could be served as a potential tool for drought tolerance determination in rice. MR219-4 and MR219-9 were found to be promising in breeding for drought tolerance as they offer better physiological adaptation to drought stress.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Amplification levels of candidate target OsLEA1, OsLEA2, OsLEA3, OsLEA4, and OsLEA5 genes in the rice genotypes under drought stress condition; Cq values indicate mean of quadruplicate samples; vertical bars represent ±standard error; values within genotype with the different letter are significantly different based on comparison using the HSD test at p ≤ 0.05.
Figure 2
Figure 2
Relative expression levels of (a) OsLEA1, (b) OsLEA2, (c) OsLEA3, (d) OsLEA4, and (e) OsLEA5 calibrated using ACT11/UBQ5 reference genes in drought stress and control rice plants by relative quantitative real-time PCR; vertical bars represent ±standard error; values within genotype with the different letter are significantly different based on comparison using the HSD test at p ≤ 0.05; symbols (∗) indicate the LEA genes were significantly different by more than 4-fold change.
See this image and copyright information in PMC

References

    1. Hong-Bo S., Zong-Suo L., Ming-An S. LEA proteins in higher plants: structure, function, gene expression and regulation. Colloids and Surfaces B: Biointerfaces. 2005;45(3-4):131–135. doi: 10.1016/j.colsurfb.2005.07.017. - DOI - PubMed
    1. Olvera-Carrillo Y., Luis Reyes J., Covarrubias A. A. Late embryogenesis abundant proteins: versatile players in the plant adaptation to water limiting environments. Plant Signaling & Behavior. 2011;6(4):586–589. doi: 10.4161/psb.6.4.15042. - DOI - PMC - PubMed
    1. Battaglia M., Covarrubias A. A. Late embryogenesis abundant (LEA) proteins in legumes. Frontiers in Plant Science. 2013;4:1–11. doi: 10.3389/fpls.2013.00190. - DOI - PMC - PubMed
    1. Goyal K., Tisi L., Basran A., et al. Transition from natively unfolded to folded state induced by desiccation in an anhydrobiotic nematode protein. Journal of Biological Chemistry. 2003;278(15):12977–12984. doi: 10.1074/jbc.M212007200. - DOI - PubMed
    1. Hu T., Zhou N., Fu M., Qin J., Huang X. Characterization of OsLEA1a and its inhibitory effect on the resistance of E.coli to diverse abiotic stresses. International Journal of Biological Macromolecules. 2016;91:1010–1017. doi: 10.1016/j.ijbiomac.2016.06.056. - DOI - PubMed

LinkOut - more resources