Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Nov;19(11):3007-3013.
doi: 10.1111/jocd.13337. Epub 2020 Feb 24.

Effects of carboxytherapy on skin laxity

Suellen Matos Domingos Oliveira  1 , Lenaldo Branco Rocha  2 , Marco Túlio Rodrigues da Cunha  3 , Mariana Molinar Mauad Cintra  3 , Nanci Mendes Pinheiro  4 , Adriana Clemente Mendonça  5
Affiliations

Effects of carboxytherapy on skin laxity

Suellen Matos Domingos Oliveira et al. J Cosmet Dermatol. 2020 Nov.

Abstract

Background: Carboxytherapy is capable of inducing tissue repair which results in an increase in elastic and collagen fibers.

Objectives: To evaluate the effects of carboxytherapy upon human skin collagen and elastic fiber synthesis.

Methods: Case study of nine volunteers who received a single application of carboxytherapy in the left infraumbilical region, with infusion rate of 100 mL/min and 0.6 mL/kg weight over an area of 25 cm2 . After 60 days on average, the skin was collected for histological analysis and stained with picrosirius red for collagen and Verhoeff for elastic fibers. The percentage of fibers found was marked by the Image J® program and recorded in a Microsoft Excel spreadsheet. Statistical analysis was performed using SPSS (version 20), with a significance level of 95%.

Results: An increase in the collagen and elastic fibers sample was observed in the treated group. Morphometrically, a significant increase in the percentage of collagen in the Carboxytherapy group (41.44 ± 4.50%) was observed compared with the control group (37.44 ± 3.87%) with P = .04; for elastic fibers, the percentage showed no significant difference between the control group (10.55 ± 4.33%) and the carboxytherapy group (10.44 ± 3.71%).

Conclusions: Carboxytherapy with the parameters used in this study was able to stimulate collagen and elastic fiber synthesis, with significant differences in the morphometry for collagen fibers.

Keywords: carbon dioxide; carboxytherapy; collagen; skin aging; skin laxity.

PubMed Disclaimer

References

REFERENCES

    1. Brandi C, D'Aniello C, Grimaldi L, et al. Carbon dioxide therapy in the treatment of localized adiposities: clinical study and histopathological correlations. Aesthetic Plast Surg. 2001;25(3):170-174.
    1. Doghaim NN, El-Tatawy RA, Neinaa YMEH, Abd El-samd MM. Study of the efficacy of carboxytherapy in alopecia. J Cosmet Dermatol. 2018;17(6):1275-1285.
    1. Varlaro V, Manzo G, Mugnaini F, Bisacci CFP. Carboxytherapy: effects on microcirculation and its use in the treatment of severe lymphedema. Acta Phlebol [Internet]. 2007;8(8):1-13.
    1. Abramo AC, Teixeira TT. Carboinsuflação em úlceras crônicas dos membros inferiores. Rev Bras Cir Plástica. 2011;26(2):205-210.
    1. Durães EFR, Durães LdC, Carneiro FP, Lino Júnior RdS, Sousa JB. The effect of carbon dioxide therapy on composite graft survival. Acta Cir Bras. 2013;28(8):589-593.

LinkOut - more resources