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. 2020 Feb 24;15(2):e0229205.
doi: 10.1371/journal.pone.0229205. eCollection 2020.

Antibiotic burden of school children from Tibetan, Hui, and Han groups in the Qinghai-Tibetan Plateau

Yushan Huang  1 , Zuhong Zhang  1 , Tianchun Hou  1 , Jingfang Shi  2 , Wenjie Huang  2 , Zhao Bai  1 , Danfeng Long  1 , Xiaodan Huang  1 , Shijuan Yan  2
Affiliations

Antibiotic burden of school children from Tibetan, Hui, and Han groups in the Qinghai-Tibetan Plateau

Yushan Huang et al. PLoS One. .

Abstract

Background: Given their geographical proximity but differences in cultural and religious dietary customs, we hypothesize that children from the three main ethnic populations (Han, Hui, and Tibetan) residing in the Qinghai-Tibetan Plateau region differs in their non-iatrogenic antibiotic loads.

Methods: To determine the antibiotic burden of the school children unrelated to medical treatment, we quantified the antibiotic residues in morning urine samples from 92 Han, 72 Tibetan, and 85 Muslim Hui primary school children aged 8 to 12 years using high-performance liquid chromatography-tandem mass spectrometry, and performed correlation analysis between these data and concurrent dietary nutrition assessments.

Results: Sixteen of the 18 targeted antibiotics (4 macrolides, 3 β-lactams, 2 tetracyclines, 4 quinolones, 3 sulfonamides, and 2 aminoanols) were identified in the urine samples with an overall detection frequency of 58.63%. The detection frequency of the six antibiotic classes ranged from 1.61% to 32.53% with ofloxacin showing the single highest frequency (18.47%). Paired comparison analysis revealed significant differences in antibiotic distribution frequency among groups, with Tibetans having higher enrofloxacin (P = 0.015) and oxytetracycline (P = 0.021) than Han children. Norfloxacin (a human/veterinary antibiotic) was significantly higher in the Hui children than in the Han children (P = 0.024). Dietary nutrient intake assessments were comparable among participants, showing adequate levels of essential vitamins and minerals across all three ethnic groups. However, significant differences in specific foods were observed among groups, notably in lower fat consumption in the Hui group.

Conclusions: The introduction and accumulation of antibiotic residues in school children through non-iatrogenic routes (food or environmental sources) poses a serious potential health risk and merits closer scrutiny to determine the sources. While the exact sources of misused or overused antibiotics remains unclear, further study can potentially correlate ethnicity-specific dietary practices with the sources of contamination.

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Distribution of mass concentration detection frequencies for 16 selected antibiotics (LOD: Limit of detection).
Fig 2
Fig 2. Detection frequency of antibiotics by antimicrobial mechanism in relation to ethnic categories (* indicates P < 0.05).
Fig 3
Fig 3
Detection frequency of antibiotic categories by antimicrobial mechanism in relation to (a) ethnic group and (b) sex. (* indicates P < 0.05).
Fig 4
Fig 4
Detection frequency of antibiotics categorized by antibiotics categorized by human treatment, veterinary treatment, or usage for either in relation to (a) ethnic group and (b) sex (* indicates P < 0.05).
See this image and copyright information in PMC

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