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. 2018;39(4):285-294.
Epub 2018 Dec 13.

Molecular Identification and Phylogenetic Analysis of Acetylcholinesterase-2 Gene From Culex pipiens And Culex quinquefasciatus (Diptera: Culicine) in Nigeria

A B Onoja  1 , M M Maina  2 , A V Opayele  1
Affiliations

Molecular Identification and Phylogenetic Analysis of Acetylcholinesterase-2 Gene From Culex pipiens And Culex quinquefasciatus (Diptera: Culicine) in Nigeria

A B Onoja et al. Niger Vet J. 2018.

Abstract

West Nile virus (WNV) is maintained in an enzootic cycle between ornithophilic mosquitoes and birds. There is paucity of information on population density of these vectors and the molecular details in Nigeria. We determined relative abundance and employed molecular methods to detect Culex species. Mosquitoes were caught from April 2013 to February 2014. Genomic DNA extraction was carried out and acetylcholinesterase 2 gene amplified by Polymerase Chain Reaction in different Culex species. Culex pipiens quinquefasciatus and Cx. p. pipiens were identified in different locations, while some other Culex species could not be identified. Culex p. quinquefasciatus were sequenced by Sanger's method. Vector density was 100 Culex species per man hour. Identification of Culex pipiens and Cx. quinquefasciatus from different locations in Ibadan, Nigeria reaffirms the presence of vectors and highlights possibility of enzootic transmission of WNV. Surveillance should be sustained in equine population and bird reservoirs.

Keywords: Culex pipiens; Culex quinquefasciatus; Nigeria.

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Conflict of interest statement

Conflict of Interest We declare that we have no conflict of interest.

Figures

Fig. 1.
Fig. 1.. Molecular identification of Cx. p. quinquefasciatus in lanes 3 and 8
Culex species were obtained from the following locations Lane 1 –Agodi, Lane 2 –Awo Hall, Lane 3 –Yemetu, Lane 4 – Department of Microbiology UI environs, Lane 5 – Negative control, Lane 6 – DNA Ladder, Lane 7 –Botanical garden UI, Lane 8 – Apata.
Fig 2.
Fig 2.. Molecular identification of Ace 2 gene of Cx. p. pipiens.
Culex speices were obtained from the following locations: Lane 1= Yemetu, Lane 2 = Awo hall, Lane 3 = Apata, Lane 4 = Botanical garden, Lane 5 = negative control, Lane 6 = Department of Microbiology environs in University of Ibadan, Lane 7 = 100bp DNA ladder and Apata.
Fig. 3.
Fig. 3.
Distribution pattern of Culex species in parts of Ibadan from April 2013 to February 2014
Fig 4.
Fig 4.
Phylogenetic tree showing sequences of Cx. p. quinquefasciatus from Apata and Yemetu in Ibadan, Nigeria.
See this image and copyright information in PMC

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References

    1. CENTERS FOR DISEASE CONTROL and PREVENTION (2008). Non pharmaceutical fentanyl-related deaths-multiple states, April 2005–March 2007. MMWR 57:793–796. - PubMed
    1. CENTERS FOR DISEASE CONTROL and PREVENTION (2010). Surveillance for human West Nile virus disease-United States, 1999–2008. MMWR 59: 2. - PubMed
    1. EASTWOOD G, KRAMER LD, GOODMAN SJ, et al., (2011). West Nile virus vector competency of Culex quinquefasciatus mosquitoes in the Galapagos Islands. Am. J. of Trop. Med. and Hyg. 85: 426–433. - PMC - PubMed
    1. ZELLER HG, SCHUFFENECKER I, (2004). West Nile virus: an overview of its spread in Europe and the Mediterranean Basin in contrast to its spread in the Americas. Eur. J. Clin. Microbiol. 23: 147–156. - PubMed
    1. PIERSON TC, DIAMOND MS, KNIPE DM, et al., (2013). Flaviviruses, editors Fields virology. 6 ed. Philadelphia: Lippincott Williams & Wilkins; pp 747–794.

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