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. 2020 Feb 10:2020:6842975.
doi: 10.1155/2020/6842975. eCollection 2020.

Establishment of an Experimental Procedure for Preparing Trial Serum Samples for the Specific Serodiagnosis of Toxocara canis for External Quality Assessment Schemes

Quang Huy Vu  1   2   3 , Diep Tuan Tran  1 , Phu Manh Sieu Tran  1   4 , Van Chuong Le  1   2 , Thi Diem Phuc Huynh  3 , Quang Sang Bui  3
Affiliations

Establishment of an Experimental Procedure for Preparing Trial Serum Samples for the Specific Serodiagnosis of Toxocara canis for External Quality Assessment Schemes

Quang Huy Vu et al. J Parasitol Res. .

Abstract

Background: External quality assessment (EQA) provides evidence of reliable, accurate, and precise results for customers using the diagnostic test for Toxocara canis. Objective. To establish a procedure for producing standard Toxocara canis serum samples for serodiagnostic testing in EQA.

Methods: The collected serum samples to contain anti-Toxocara canis serum samples for serodiagnostic testing in EQA. F-test and a T-test were applied to evaluate their homogeneity and stability.

Results: Among eleven samples positive by ELISA, ten of them were confirmed via Western blotting by positive reaction with 5 specific Toxocara canis serum samples for serodiagnostic testing in EQA. Toxocara canis serum samples for serodiagnostic testing in EQA. p > 0.05). Samples produced by both methods were stable for 7 days at 30°C (p > 0.05). Samples produced by both methods were stable for 7 days at 30°C (.

Conclusion: Specific serodiagnosis samples of anti-Toxocara canis antibodies for EQA could be produced that possessed homogeneity and stability lasting for 3 months and 6 months by the freeze-drying and freezing methods, respectively. At 30°C, the samples produced by both methods were stable for 7 days, suitable for delivery to remote laboratories.Toxocara canis serum samples for serodiagnostic testing in EQA.

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Conflict of interest statement

The authors declare no conflict of interest, financial, or otherwise.

Figures

Figure 1
Figure 1
Protein bands detected by Western blotting that correspond to Toxocara canis antigens. Strips 1 and 2 were the positive and negative controls, respectively, while strips 3 to 12 represent positive serum samples.
Figure 2
Figure 2
The workflow of preparation of trial samples containing an anti-Toxocara canis antibody used in EQA.
See this image and copyright information in PMC

References

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