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. 2020 Aug;108(6):2610-2620.
doi: 10.1002/jbm.b.34593. Epub 2020 Feb 25.

Biological evaluation of zinc-containing calcium alginate-hydroxyapatite composite microspheres for bone regeneration

Renan C Cuozzo  1 , Suelen C Sartoretto  2   3 , Rodrigo F B Resende  3   4 , Adriana Terezinha N N Alves  5 , Elena Mavropoulos  6 , Marcelo H Prado da Silva  7 , Mônica D Calasans-Maia  4
Affiliations

Biological evaluation of zinc-containing calcium alginate-hydroxyapatite composite microspheres for bone regeneration

Renan C Cuozzo et al. J Biomed Mater Res B Appl Biomater. 2020 Aug.

Abstract

Zinc is an important element for bone structure and metabolism. Its interaction with hydroxyapatite has been investigated for the improvement of bone repair. The objective of this study was to evaluate the in vitro and in vivo biological response to nanostructured calcium alginate-hydroxyapatite (HA) and zinc-containing HA (ZnHA). Cytocompatibility was evaluated by applying PrestoBlue reagent after exposing murine pre-osteoblast cells to extracts of each biomaterial microspheres. After physical and chemical characterization, the biomaterial microspheres were implanted in a critical size calvaria defect (8 mm) in Wistar rats (n = 30) that were randomly divided into the HA and ZnHA groups. Tissue samples were evaluated through histological and histomorphometric analyses after 1, 3, and 6 months (n = 5). The results showed cellular viability for both groups compared to the negative control, and no differences in metabolic activity were observed. The HA group presented a significant reduction of biomaterial compared with the ZnHA group in all experimental periods; however, a considerable amount of new bone formation was observed surrounding the ZnHA spheres at the 6-month time point compared with the HA group (p < .05). Both biomaterials were biocompatible, and the combination of zinc with hydroxyapatite was shown to improve bone repair.

Keywords: alginate; bone repair; hydroxyapatite; in vitro; in vivo; zinc.

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References

REFERENCES

    1. Bose, S., & Tarafder, T. (2012). Calcium phosphate ceramic systems in growth factor and drug delivery for bone tissue engineering: A review. Acta Biomaterialia, 8, 1401-1421.
    1. Calasans-Maia, M., Calasans-Maia, J., Santos, S., Mavropoulos, E., Farina, M., Lima, I., … Granjeiro, J. M. (2014). Short-term in vivo evaluation of zinc-containing calcium phosphate using a normalized procedure. Materials Science & Engineering C Materials for Biological Applications, 41, 309-319.
    1. Calasans-Maia, M., Fernandes, G. V. O., Rossi, A., Dias, E. P., Almeida, G. D. S., Mitri, F. F., & Granjeiro, J. M. (2008). Effect of hydroxyapatite and zinc-containing hydroxyapatite on osseous repair of critical size defect in the rat calvaria. Key Engineering Materials, 361-363, 1273-1276.
    1. Cammack, G. V., Nevins, M., Clem, D. S., Hatch, J. P., & Mellonig, J. T. (2005). Histologic evaluation of mineralized and desmineralized freeze dried bone allograft for ridge and sinus augmentations. International Journal of Periodontics Restorative Dentistry, 25, 231-237.
    1. Cruz, R., Calasans Maia, M., Calasans Maia, J., Sartoretto, S., Moraschini, V., Rossi, A., … Granjeiro, J. M. (2018). Does the incorporation of zinc into calcium phosphate improve bone repair? A systematic review. Ceramics International, 44, 1240-1249.