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. 2020 Feb 22;10(2):385.
doi: 10.3390/nano10020385.

Functional Bioglass-Biopolymer Double Nanostructure for Natural Antimicrobial Drug Extracts Delivery

Irina Negut  1 , Laura Floroian  2 , Carmen Ristoscu  1 , Cristian N Mihailescu  1 , Julia Claudia Mirza Rosca  3 , Tatiana Tozar  1 , Mihaela Badea  4 , Valentina Grumezescu  1 , Claudiu Hapenciuc  1 , Ion N Mihailescu  1
Affiliations

Functional Bioglass-Biopolymer Double Nanostructure for Natural Antimicrobial Drug Extracts Delivery

Irina Negut et al. Nanomaterials (Basel). .

Abstract

Aseptic loosening and periprosthetic infections are the main causes of implant failure. Strategies to mitigate this drawback are therefore mandatory to avoid primary and revision replacement surgeries. A functional bioapatite-biopolymer double nanostructure fabricated by matrix-assisted pulsed laser evaporation to prevent infection of orthopedic and dental implants could promote osseointegration and ensure controlled delivery of natural antimicrobial drugs. The synthesized nanostructure consists of two overlapping layers, the lower from a biocompatible polymer for anticorrosive protection, and the upper of bioactive glass incorporating antimicrobial plant extract, acting as a potential drug delivery system. Morphology, composition, adherence, ability for drug delivery and biological properties (cytotoxicity and antimicrobial effect) were studied. Structures proved compact and stable, conserving a remarkable drug delivery ability for more than 21 days, i.e., enough to ensure long-term microbes' eradication.

Keywords: antimicrobial effect; bioactive coatings; drug release; implant protection.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Typical SEM micrographs for BGN/PMMA coated substrates: (a) overview, (b) detail, (c) cross section.
Figure 2
Figure 2
FTIR spectrum of BGN/PMMA/stainless steel (SS) film deposited by matrix-assisted pulsed laser evaporation (MAPLE) and of single components, namely Neem, BG, and PMMA.
Figure 3
Figure 3
AFM images of films surface: (a) BGN/PMMA/Ti, (b) BGN/PMMA/SS.
Figure 4
Figure 4
Innitial delamination (a) and first coating detachment (b) from SS substrate.
Figure 5
Figure 5
Profilometric measurement on BGN/PMMA/SS sample and mean wear track section (red field).
Figure 6
Figure 6
SEM images of (a) BGN/PMMA /Ti and (b) BGN/PMMA/SS thin films after 28 days of immersion in SBF, at different magnifications
Figure 7
Figure 7
AFM images of thin films after immersion in SBF: (a) BGN/PMMA/Ti, (b) BGN/PMMA/SS.
Figure 8
Figure 8
Drug release as function of time for BGN/PMMA/SS (black curve) and BGN/PMMA/Ti (red curve): (a) peak absorbance intensity function of time and (b) concentration function of time.
Figure 9
Figure 9
FTIR spectra of the initial and immersed samples for different time, respectively.
Figure 10
Figure 10
Bode diagrams for BGN/PMMA/SS (a) and SS (b) samples after different immersion time.
Figure 11
Figure 11
Impedance plots for BGN/PMMA/SS (a) and SS (b) samples after different immersion time.
Figure 12
Figure 12
Cytotoxicity test results.
Figure 13
Figure 13
Fluorescence microscopy images of SaOs2 cells labeled with Ki67 and DAPI after cultivation on BGN/PMMA/SS (a) and SS (b) samples. 100X magnification.
Figure 14
Figure 14
Proliferation of cells at passage 9 after 6 days from seeding on BGN/PMMA/SS and bare SS samples.
See this image and copyright information in PMC

References

    1. Kumar M., Kumar R., Kumar S., Prakash C. Biomechanical Properties of Orthopedic and Dental Implants: A Comprehensive Review. Handb. Res. Green Eng. Tech. Mod. Manuf. 2019:1–13. doi: 10.4018/978-1-5225-5445-5.ch001. - DOI
    1. Scholz M.S., Blanchfield J.P., Bloom L.D., Coburn B.H., Elkington M., Fuller J.D., Gilbert M.E., Muflahi S.A., Pernice M.F., Trevarthen J.A., et al. The use of composite materials in modern orthopaedic medicine and prosthetic devices: A review. Compos. Sci. Technol. 2011;71:1791–1803. doi: 10.1016/j.compscitech.2011.08.017. - DOI
    1. Kaur G., Kumar V., Baino F., Mauro J.C., Pickrell G., Evans I., Bretcanu O. Mechanical properties of bioactive glasses, ceramics, glass-ceramics and composites: State-of-the-art review and future challenges. Mater. Sci. Eng. C. 2019;104:109895. doi: 10.1016/j.msec.2019.109895. - DOI - PubMed
    1. Vukajlovic D., Parker J., Bretcanu O., Novakovic K. Chitosan based polymer/bioglass composites for tissue engineering applications. Mater. Sci. Eng. C. 2019;96:955–967. doi: 10.1016/j.msec.2018.12.026. - DOI - PubMed
    1. Floroian L., Samoila C., Badea M., Munteanu D., Ristoscu C., Sima F., Negut I., Chifiriuc M.C., Mihailescu I.N. Stainless steel surface biofunctionalization with PMMA-bioglass coatings: Compositional, electrochemical corrosion studies and microbiological assay. J. Mater. Sci. Mater. Med. 2015;26:195. doi: 10.1007/s10856-015-5527-y. - DOI - PubMed

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