Dataset on optimization of lignin peroxidase production by Endomelanconiopsis sp. under submerged fermentation using one factor at a time approach

Abstract

One factor at a time approach was employed for the optimization of media components, physical factors and additional additives for lignin peroxidase (LiP) production under submerged fermentation by the endophytic fungus Endomelanconiopsis sp. The optimized conditions derived were: carbon source (dextrose 10 g/L), nitrogen source (ammonium tartarate 0.027 g/L), veratryl alcohol (1 mM), MnSO₄ (0.5 g/L), inoculum concentration (3 mycelial plugs), pH (5), temperature (30 °C) and Tween 80 (1.5%). A 40 fold increase of enzyme activity was observed after the single parameter optimization. The lignin peroxidase (LiP) production to the level of 345.26 ± 0.52 IU/mL indicates that the fungus has the commercial potential for LiP.

Keywords: Endomelanconiopsis sp.; Lignin peroxidase; One factor at a time method; Optimization.

Fig. 1

Effect of carbon source (dextrose) on LiP production from Endomelanconiopsis sp.

Fig. 2

Effect of nitrogen source (ammonium tartarate) on LiP production from Endomelanconiopsis sp.

Fig. 3

Effect of various factors on LiP production from Endomelanconiopsis sp.: a) veratryl alcohol, b) MnSO₄, c) inoculum concentration d) pH, e) temperature.

Fig. 4

Effect of various factors on LiP production from Endomelanconiopsis sp.: a) detergents, b) tween 80.

Fig. 5

Time course analysis under optimized condition for LiP production.