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. 2020 Mar 19;382(12):1175-1177.
doi: 10.1056/NEJMc2000231. Epub 2020 Feb 26.

An Animal Model of Inhaled Vitamin E Acetate and EVALI-like Lung Injury

Tariq A Bhat  1 , Suresh G Kalathil  1 , Paul N Bogner  1 , Benjamin C Blount  2 , Maciej L Goniewicz  3 , Yasmin M Thanavala  3
Affiliations

An Animal Model of Inhaled Vitamin E Acetate and EVALI-like Lung Injury

Tariq A Bhat et al. N Engl J Med. .
No abstract available

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Figures

Figure 1.
Figure 1.. Findings from a Mouse Model of Electronic-Cigarette, or Vaping, Product Use–Associated Lung Injury (EVALI).
Panel A shows levels of vitamin E acetate (VEA) quantified by isotope-dilution mass spectrometry in bronchoalveolar-lavage (BAL) fluid harvested from mice. Values are means and standard deviations for 10 mice. Panel B shows albumin levels measured in BAL fluid from mice exposed to air, a mixture of propylene glycol and vegetable glycerin (PG–VG), or VEA. Values are means and standard deviations for 10 mice. Panel C shows the total number of CD45+ cells infiltrating the lung in mice exposed to air, PG–VG, or VEA. Values are means and standard deviations for 10 mice. The P values in Panels A, B, and C were calculated by two-way analysis of variance in Tukey’s post-test comparisons among the exposure groups. Panel D shows BAL fluid from a mouse exposed to VEA, containing lipid-laden macrophages (representative examples are indicated with arrows) with cytoplasmic staining by oil red O in a vesicular pattern. The macrophages are numerous and contain variable amounts of lipid. Background pneumocytes (arrowheads) show comparatively scant cytoplasm and are present as single cells or loose sheets. Panel E shows BAL fluid from a mouse exposed to PG–VG, which contained fewer identifiable macrophages and had minimal to no specific staining by oil red O. Without lipid staining, it is more difficult to distinguish between small alveolar macrophages and pneumocytes in these preparations. Panels F and G show findings in lung sections. In mice exposed to VEA (Panel F), alveolar macrophages (arrowheads and circles) in residence among pneumocytes (P) lining the alveoli (A) contained abundant oil red O–stained lipid. In mice exposed to PG–VG, tiny oil red O–stained granules in the cytoplasm of cells lining the alveoli, including pneumocytes (arrows) and alveolar macrophages (arrowheads), were observed. B denotes bronchiole.
See this image and copyright information in PMC

References

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