Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Feb 25;30(8):2463-2471.e5.
doi: 10.1016/j.celrep.2020.01.096.

Highly Parallel Quantification and Compartment Localization of Transcription Factors and Nuclear Proteins

Alexander J Federation  1 , Vivek Nandakumar  1 , Brian C Searle  2 , Andrew Stergachis  2 , Hao Wang  1 , Lindsay K Pino  2 , Gennifer Merrihew  2 , Ying S Ting  2 , Nicholas Howard  1 , Tanya Kutyavin  1 , Michael J MacCoss  3 , John A Stamatoyannopoulos  4
Affiliations
Free article

Highly Parallel Quantification and Compartment Localization of Transcription Factors and Nuclear Proteins

Alexander J Federation et al. Cell Rep. .
Free article

Abstract

Transcription factors and other chromatin-associated proteins are difficult to quantify comprehensively. Here, we combine facile nuclear sub-fractionation with data-independent acquisition mass spectrometry to achieve rapid, sensitive, and highly parallel quantification of the nuclear proteome in human cells. We apply this approach to quantify the response to acute degradation of BET bromodomains, revealing unexpected chromatin regulatory dynamics. The method is simple and enables system-level study of previously inaccessible chromatin and genome regulators.

Keywords: chromatin; data-independent acquisition; proteomics; small molecule degredation; transcription factors.

PubMed Disclaimer

Conflict of interest statement

Declaration of Interests The MacCoss Lab at the University of Washington has a sponsored research agreement with Thermo Fisher Scientific, the manufacturer of the instrumentation used in this research. Additionally, M.J.M. is a paid consultant for Thermo Fisher Scientific. A patent application has been filed on the methods described.

Publication types

LinkOut - more resources