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. 2020 Feb 1;21(2):295-300.
doi: 10.31557/APJCP.2020.21.2.295.

Activation of MAPK and Cyclin D1/CDK4 in Malignant Transformation of Human Embryonic Lung Fibroblasts Induced by Silica and Benzopyrene

Huan Wang  1 , Shuyu Xiao  2 , Yali Tang  1 , Ke Han  1 , Zheng Zhang  1 , Yulan Jin  1 , Fuhai Shen  1
Affiliations

Activation of MAPK and Cyclin D1/CDK4 in Malignant Transformation of Human Embryonic Lung Fibroblasts Induced by Silica and Benzopyrene

Huan Wang et al. Asian Pac J Cancer Prev. .

Abstract

Objective: Silica and Benzo(a)pyrene are listed as carcinogens. This study aims to explore Cyclin D1, CDK4 and difference of cell cycle adjusted by MAPK signal transduction pathway in silica and B(a)P-induced malignant transformation of human embryonic lung fibroblasts.

Methods: Activity of the subfamily (ERK, p38 and JNK) of mitogen-activated protein kinase (MAPK), cyclin D1 and CDK4 (cyclin dependent kinase) were evaluated using Human embryonic lung fibroblast (HELF) purchased from the cell room, basic research institute, Chinese Academy of Medical Sciences. The expression of cyclin D1 and CDK4 (cyclin dependent kinase) were measured in silica and B(a)P induced malignant using Western blot (WB) assay.

Result: P-ERK and P-JNK expression increased significantly (P<0.01), while CDK4 and P-p38 expression decreased (P<0.01, P<0.05) in silica-induced malignant transformation cells compared with the control group. P-ERK, P-JNK and Cyclin D1 expression increased (P<0.01, P<0.01, P<0.05) in B(a)P-induced group compared with the control group. P-ERK and P-JNK expression decreased (P<0.01), while P-p38, Cyclin D1 and CDK4 expression increased (P<0.05, P<0.05, P<0.01) in B(a)P-induced group compared with the silica-induced group.

Conclusion: MAPK and cyclin D1/CDK4 activation expressed differently in human embryo lung fibroblasts malignant transformation induced by silica and benzopyrene.

Keywords: B(a) P; Cyclin D1; HELF; MAPK; Silica.

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Figures

Figure 1
Figure 1
The Cyclin D1 and CDK4 Expressed in Malignant Transformation HELF Induced by Silica and B(a)P
Figure 2
Figure 2
The Total Levels of ERK, p38, JNK Expressed in Malignant Transformation HELF Induced by Silica and B(a)P
Figure.3
Figure.3
The Phosphrylation of ERK, p38, JNK, Cyclin D1 and CDK4 Expressed in Malignant Transformation HELF Induced by Silica and B(a)P Cyclin D1 and CDK4 (Figure 1), ERK, p38 and JNK (Figure 2), P-ERK, P-p38 and P-JNK (Figure 3) were expressed in malignant transformation HELF induced by silica and B(a)P. Each experiment was conducted at least thrice in duplicate. Error bars indicate standard deviation(SD). *P< 0.05, compared with control group. **P< 0.01, compared with control group. #P< 0.05, compared with silica transformation group. ##P < 0.01, compared with silica transformation group
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