Gorillibacterium timonense sp. nov., isolated from an obese patient

Abstract

A Gram-negative and facultative anaerobic bacterium, designated strain SN4^T, was isolated from the stool sample of an obese Amazonian patient. The new isolate was characterized by the taxonogenomics approach. The strain SN4^T was beige-colored, circular and not haemolytic. Cells are rod shaped and motile with several flagella. Strain SN4^T grows optimally at pH 7 and can survive in the presence of a saline concentration of up to 75 g/l NaCl. The 16S ribosomal RNA gene sequence analysis of the novel strain SN4^T showed 95.28% similarity in nucleotide sequence with Gorillibacterium massiliense G5^T, the phylogenetically closest neighbor and the type species of this genus. Anteiso-C_15:0, iso-C_15:0 and C_16:0 were found as the major components in the cellular fatty acid analysis of this isolate. The genomic draft of strain SN4^T is 5,263,742 bp long with 53.33% of G+C content. The differences in physiological, biochemical characteristics and phylogenetic and genomic data make it possible to clearly distinguish the strain SN4^T from G. massiliense G5^T. Based on the taxonogenomic description and the phenotypic and biochemical characteristics of this bacterium presented in this article, we propose the SN4^T strain (= CSUR P2011 = DSM 100,698) as a new species, Gorillibacterium timonense sp. nov.

Keywords: Genome; Gorillibacterium timonense; Human gut microbiota; Taxonogenomics.