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. 2019;5(4):43-49.
doi: 10.18502/cmm.5.4.2149.

Molecular identification of Fusarium species complex isolated from clinical samples and its antifungal susceptibility patterns

Yashik Bansal  1 , Nidhi Singla  1 , Neelam Kaistha  1 , Sunandan Sood  2 , Jagdish Chander  1
Affiliations

Molecular identification of Fusarium species complex isolated from clinical samples and its antifungal susceptibility patterns

Yashik Bansal et al. Curr Med Mycol. 2019.

Abstract

Background and purpose: More than 300 Fusarium species are grouped into approximately 23 species complexes out of which around 70 are involved in human infections. The nomenclature of these species has undergone considerable changes in recent years. These species cause localized infections in individuals while inducing systemic infections mainly in immunocompromised patients. The present study was conducted to identify Fusarium species in clinical isolates by molecular methods and determine their in vitro minimum inhibitory concentration (MIC) patterns to address the lack of data in this domain in Northern India.

Materials and methods: For the purpose of the study, Fusarium isolates obtained from various clinical samples were sent to the Westerdijk Fungal Biodiversity Institute, Utrecht, the Netherlands, for molecular identification. The MIC testing was performed using the microbroth dilution method as per the Clinical and Laboratory Standards Institute reference method (M38-A2).

Results: Fusarium was isolated from 33 patients (i.e., 1, 1, 2, 14, and 15 cases with endophthalmitis, sinusitis, pulmonary involvement, onychomycosis, and keratitis, respectively). These 33 isolates belonged to three species complexes, namely F. solani species complex (FSSC; n=13), F. fujikuroi species complex (FFSC; n=13), and F. incarnatum equiseti species complex (FIESC; n=7). The species identified within FSSC, FFSC, and FIESC included F. keratoplasticum (n=6)/F. falciforme (n=6)/F. solani (n=1), F. proliferatum (n=7)/F. sacchari (n=5)/F. anthophilum (n=1), and F. incarnatum SC species (n=6)/F. equiseti SC species (n=1), respectively. The MIC results showed that all isolates had a lower MIC against amphotericin B than against the other antifungal agents.

Keywords: Fusarium; Keratitis; Molecular identification; Onychomycosis; Taxonomy.

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References

    1. Costa-Orlandi CB, Sardi JCO, Pitangui NS, de Oliveira HC, Scorzoni L, Galeane MC, et al. Fungal biofilms and polymicrobial diseases. J Fungi (Basel) 2017;3(2) - PMC - PubMed
    1. Nucci M, Anaissie EJ. Fusarium infections in immune-compromised patients. Clin Micro Rev. 2007;20(4):695–704. - PMC - PubMed
    1. Tupaki-Sreepurna A, Kindo AJ. Fusarium: the versatile pathogen. Indian J Med Microbiol. 2018;36(1):8–17. - PubMed
    1. Moretti ML, Busso-Lopes AF, Tararam CA, Moraes R, Muraosa Y, Mikami Y, et al. Airborne transmission of invasive fusariosis in patients with hematologic malignancies. PloS One. 2018;13(4):e0196426. - PMC - PubMed
    1. Chilaka CA, De Boevre M, Atanda OO, De Saeger S. The status of Fusarium mycotoxins in Sub-Saharan Africa: a review of emerging trends and post-harvest mitigation strategies towards food control. Toxins (Basel) 2017;9(1):E19. - PMC - PubMed

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