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. 2020 Feb 28;9(1):496-504.
doi: 10.1080/22221751.2020.1730710. eCollection 2020.

Vector competence of anthropophilic mosquitoes for a new mesonivirus in Senegal

Alioune Gaye  1 , Moussa Moïse Diagne  2 , El Hadji Ndiaye  1 , Marie Henriette Dior Ndione  2   3 , Martin Faye  2 , Cheikh Talla  4 , Gamou Fall  2 , Yamar Ba  1 , Diawo Diallo  1 , Ibrahima Dia  1 , Pascal Handschumacher  5 , Ousmane Faye  2 , Amadou Alpha Sall  2 , Mawlouth Diallo  1
Affiliations

Vector competence of anthropophilic mosquitoes for a new mesonivirus in Senegal

Alioune Gaye et al. Emerg Microbes Infect. .

Abstract

The mesoniviruses (MESOVs) belong to the newly described Mesoniviridae family (Order: Nidovirales). They have never been reported in Senegal until recently during a study in arbovirus emergence with the detection of a new species of MESOV named Dianke virus (DKV) from common mosquitoes from eastern Senegal. Actually, their vector competence for this newly described DKV is unknown. We, therefore, estimated the vector competence of Culex tritaeniorhynchus, Culex quinquefasciatus, Aedes aegypti, and Anopheles gambiae mosquitoes collected in Senegal for DKV using oral infection. Whole bodies, legs/wings, and saliva samples were tested for DKV by RT-PCR to estimate infection, dissemination, and transmission rates. The infectivity of virus particles in the saliva was confirmed by infecting C6/36 cells. Virus transmission rates were up to 95.45% in Culex tritaeniorhynchus, 28% in Cx. quinquefasciatus and 9.09% in Aedes aegypti. Viral particles in the saliva were confirmed infectious by C6/36 cell culture. An. gambiae was able to disseminate DKV only at 20 days post-infection. This study shows that Culex mosquitoes are more competent than Ae. aegypti for DKV, while Anopheles gambiae is not likely a competent vector.

Keywords: Aedes; Anopheles; Culex; Dianke virus; Vector competence.

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Conflict of interest statement

No potential conflict of interest was reported by the author(s).

Figures

Figure 1.
Figure 1.
Infection, Dissemination and Transmission rates at 1, 2, 3 … 20 dpi for Ae. aegypti and An. gambiae orally exposed to DKV. Errors bar represent the upper limits of the 95% confidence intervals.
Figure 2.
Figure 2.
Infection, Dissemination and Transmission rates at 7, 10 and 15 dpi for Cx. tritaeniorhynchus and at 15 dpi for Cx. quinquefasciatus orally exposed to DKV. Errors bar represent the upper limits of the 95% confidence intervals.
Figure 3.
Figure 3.
The intervals (min-max) of numbers of RNA copies of DKV in saliva (black line) and post-infection C6/36 cells cultures (red line).
Figure 4.
Figure 4.
The risk factors for mosquito infection.
Figure 5.
Figure 5.
The risk factors for mosquito dissemination.
Figure 6.
Figure 6.
The risk factors for mosquito transmission.
See this image and copyright information in PMC

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