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. 2020 Jun;92(6):618-631.
doi: 10.1002/jmv.25736. Epub 2020 Mar 5.

Development of epitope-based peptide vaccine against novel coronavirus 2019 (SARS-COV-2): Immunoinformatics approach

Manojit Bhattacharya  1   2 , Ashish R Sharma  1 , Prasanta Patra  2 , Pratik Ghosh  2 , Garima Sharma  3 , Bidhan C Patra  2 , Sang-Soo Lee  1 , Chiranjib Chakraborty  1   4
Affiliations

Development of epitope-based peptide vaccine against novel coronavirus 2019 (SARS-COV-2): Immunoinformatics approach

Manojit Bhattacharya et al. J Med Virol. 2020 Jun.

Abstract

Recently, a novel coronavirus (SARS-COV-2) emerged which is responsible for the recent outbreak in Wuhan, China. Genetically, it is closely related to SARS-CoV and MERS-CoV. The situation is getting worse and worse, therefore, there is an urgent need for designing a suitable peptide vaccine component against the SARS-COV-2. Here, we characterized spike glycoprotein to obtain immunogenic epitopes. Next, we chose 13 Major Histocompatibility Complex-(MHC) I and 3 MHC-II epitopes, having antigenic properties. These epitopes are usually linked to specific linkers to build vaccine components and molecularly dock on toll-like receptor-5 to get binding affinity. Therefore, to provide a fast immunogenic profile of these epitopes, we performed immunoinformatics analysis so that the rapid development of the vaccine might bring this disastrous situation to the end earlier.

Keywords: SARS-COV-2; epitopes; immunoinformatics; vaccine.

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Figures

Figure 1
Figure 1
Graphical representation of linear B‐cell epitopes within the spike glycoprotein of SARS‐COV‐2
Figure 2
Figure 2
Tertiary structural model of construct vaccine component
Figure 3
Figure 3
Different molecular characterization of vaccine model. (a) All atoms at Ramachandran plot, (b) “Z” score plot of vaccine model in ProSA server, and (c) all residue energy plot
Figure 4
Figure 4
Docking complex exhibiting the surface interaction between vaccine component (cyan color) and toll‐like receptor‐5 (green color)
Figure 5
Figure 5
Docking complex exhibiting the bonding interaction between vaccine component and toll‐like receptor‐5
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