The aging effects on phenylephrine-induced relaxation of bladder in mice
- PMID: 32110516
- PMCID: PMC7015013
- DOI: 10.4103/tcmj.tcmj_178_18
The aging effects on phenylephrine-induced relaxation of bladder in mice
Abstract
Objective: We have demonstrated that phenylephrine (PE) activates the capsaicin-sensitive nerves, and then activates capsaicin-sensitive nerves to release an unknown substance that facilitates the release of norepinephrine (NE) from adrenergic nerves. Subsequently, NE stimulates β-ARs in the detrusor muscle in mice, leading to neurogenic relaxation of the urinary bladder (UB).
Materials and methods: We examined if there existed sensory-motor dysfunction in UB of aging mice. To investigate the change of PE-induced detrusor relaxation in aging male-C57BL/6 mice (12- vs. 24-month-old mice), UB strips from mice were isolated, cut into strips, and mounted in the organ bath.
Results: The UB strip contractility responding to various agents was estimated using tissue bath wire myography. Acetylcholine (ACh) and KCl-induced UB strips contraction was not significantly different between 24- and 12-month mice. NE-induced UB strips relaxation was significantly lower in 24-month than 12-month mice. Denuded bladder strips showed similar decreased relaxation response to NE. This NE-induced relaxation was inhibited by silodosin and lidocaine. PE did not induce contraction in UB strips of aging mice. In contrast, PE-induced relaxation was weaker in 24-month than 12-month mice.
Conclusion: Our results suggested that the PE-induced relaxation was age related. Aging seemed to lead the sensory-motor dysfunction. More animal and human studies are required to prove this concept and its clinical usefulness in the future.
Keywords: Adrenergic receptor; Aging; Bladder; Lower urinary tract dysfunction; Sensory-motor interaction.
Copyright: © 2019 Tzu Chi Medical Journal.
Conflict of interest statement
There are no conflicts of interest.
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References
-
- Edvardsen P. Nervous control of urinary bladder in cats I The collecting phase. Acta Physiol Scand. 1968;72:157–71. - PubMed
-
- Derweesh IH, Wheeler MA, Weiss RM. Alterations in G-proteins and beta-adrenergic responsive adenylyl cyclase in rat urinary bladder during aging. J Pharmacol Exp Ther. 2000;294:969–74. - PubMed
-
- Ordway GA, Kolta MG, Gerald MC, Wallace LJ. Age-related change in alpha-adrenergic responsiveness of the urinary bladder of the rat is regionally specific. Neuropharmacology. 1986;25:1335–40. - PubMed
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