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. 2020 Feb 20:14:160-164.
doi: 10.1016/j.reth.2020.01.002. eCollection 2020 Jun.

Effect of cell seeding methods on the distribution of cells into the gelatin hydrogel nonwoven fabric

Kumiko Matsuno  1   2   1   1   2   2 , Toshiki Saotome  1   2   1   1   2   2 , Naoki Shimada  1   1   2   2 , Koichiro Nakamura  1   2   2 , Yasuhiko Tabata  2
Affiliations

Effect of cell seeding methods on the distribution of cells into the gelatin hydrogel nonwoven fabric

Kumiko Matsuno et al. Regen Ther. .
No abstract available

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Figures

Fig. 1
Fig. 1
Number and distribution of cells in GHNF 3 h after seeding or further cultivation for 24 and 72 h by the rotary culture. (A) A whole images of cross sections (Magnification: 20×, scale bar: 1 mm) (B) Detail images of cross sections (Magnification: 20×, scale bar: 50 μm). The black arrows indicate the seeded cells. (C) Time course of cell proliferation and distribution in the outer (□) or inner portion (■) of GHNF after culture for different time periods. *, p < 0.05; significant difference against the number of the cells initially attached in the outer portion. †, p < 0.05; significant difference against the number of the cells initially attached in the inner portion. ‡, p < 0.05; significant difference between numbers in the outer portion at the same time point. §, p < 0.05; significant difference between numbers in the inner portion at the same time point. ||, p < 0.05; significant difference between numbers in the total cell numbers at the same time point.
Fig. 2
Fig. 2
The number of cells proliferated in GHNF for 3 h as a function of pre-swelling rate. *, p < 0.05; significant difference between the two groups.
Fig. 3
Fig. 3
The LDH activity of cells proliferated in GHNF for 3 h as a function of pre-swelling rate. *, p < 0.05; significant difference between the two groups.
See this image and copyright information in PMC

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