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. 2020 Feb 20:14:160-164.
doi: 10.1016/j.reth.2020.01.002. eCollection 2020 Jun.

Effect of cell seeding methods on the distribution of cells into the gelatin hydrogel nonwoven fabric

Kumiko Matsuno  1   2   1   1   2   2 , Toshiki Saotome  1   2   1   1   2   2 , Naoki Shimada  1   1   2   2 , Koichiro Nakamura  1   2   2 , Yasuhiko Tabata  2
Affiliations

Effect of cell seeding methods on the distribution of cells into the gelatin hydrogel nonwoven fabric

Kumiko Matsuno et al. Regen Ther. .
No abstract available

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Figures

Fig. 1
Fig. 1
Number and distribution of cells in GHNF 3 h after seeding or further cultivation for 24 and 72 h by the rotary culture. (A) A whole images of cross sections (Magnification: 20×, scale bar: 1 mm) (B) Detail images of cross sections (Magnification: 20×, scale bar: 50 μm). The black arrows indicate the seeded cells. (C) Time course of cell proliferation and distribution in the outer (□) or inner portion (■) of GHNF after culture for different time periods. *, p < 0.05; significant difference against the number of the cells initially attached in the outer portion. †, p < 0.05; significant difference against the number of the cells initially attached in the inner portion. ‡, p < 0.05; significant difference between numbers in the outer portion at the same time point. §, p < 0.05; significant difference between numbers in the inner portion at the same time point. ||, p < 0.05; significant difference between numbers in the total cell numbers at the same time point.
Fig. 2
Fig. 2
The number of cells proliferated in GHNF for 3 h as a function of pre-swelling rate. *, p < 0.05; significant difference between the two groups.
Fig. 3
Fig. 3
The LDH activity of cells proliferated in GHNF for 3 h as a function of pre-swelling rate. *, p < 0.05; significant difference between the two groups.
See this image and copyright information in PMC

References

    1. Li Y., Ma T., Kniss D.A., Lasky L.C., Yang S.T. Effects of filtration seeding on cell density, spatial distribution, and proliferation in nonwoven fibrous matrices. Biotechnol Prog. 2001;17:935–944. - PubMed
    1. Ratheesh G., Venugopal J.R., Chinappan A., Ezhilarasu H., Sadiq A., Ramakrishna S. 3D fabrication of polymeric scaffolds for regenerative therapy. ACS Biomater Sci Eng. 2017;3:1175–1194. - PubMed
    1. Haycock J.W. vol. 695. 2011. (3D cell culture: a review of current approaches and techniques). - PubMed
    1. Hosseinkhani H., Inatsugu Y., Hiraoka Y., Inoue S., Tabata Y. Perfusion culture enhances osteogenic differentiation of rat mesenchymal stem cells in collagen sponge reinforced with poly (glycolic acid) fiber. Tissue Eng. 2005;11:1476–1488. - PubMed
    1. An J., Teoh J.E.M., Suntornnond R., Chua C.K. Design and 3D printing of scaffolds and tissues. Engineering. 2015

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