Comparison of different samples for 2019 novel coronavirus detection by nucleic acid amplification tests

Chunbao Xie¹, Lingxi Jiang¹, Guo Huang¹, Hong Pu², Bo Gong³, He Lin¹, Shi Ma¹, Xuemei Chen⁴, Bo Long⁵, Guo Si⁵, Hua Yu¹, Li Jiang¹, Xingxiang Yang⁶, Yi Shi⁷, Zhenglin Yang⁸

Affiliations

¹ Sichuan Provincial Key Laboratory for Human Disease Gene Study and Institute of Laboratory Medicine, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.
² Department of Radiology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.
³ Sichuan Provincial Key Laboratory for Human Disease Gene Study and Institute of Laboratory Medicine, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China; Institute of Biology, Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, Chengdu, Sichuan, China.
⁴ Department of Infectious Disease, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.
⁵ Department of Infectious Disease, Sichuan Mianyang 404 Hospital, Mianyang, China.
⁶ Department of Infectious Disease, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China. Electronic address: 350686908@qq.com.
⁷ Sichuan Provincial Key Laboratory for Human Disease Gene Study and Institute of Laboratory Medicine, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China; Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences (2019RU026), Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, Sichuan, China. Electronic address: shiyi1614@126.com.
⁸ Sichuan Provincial Key Laboratory for Human Disease Gene Study and Institute of Laboratory Medicine, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China; Institute of Biology, Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, Chengdu, Sichuan, China; Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences (2019RU026), Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, Sichuan, China. Electronic address: zhenglin.yang@hsc.utah.edu.

PMID: 32114193
PMCID: PMC7129110
DOI: 10.1016/j.ijid.2020.02.050

Comparison of different samples for 2019 novel coronavirus detection by nucleic acid amplification tests

Chunbao Xie et al. Int J Infect Dis. 2020 Apr.

. 2020 Apr:93:264-267.

doi: 10.1016/j.ijid.2020.02.050. Epub 2020 Feb 27.

Authors

Chunbao Xie¹, Lingxi Jiang¹, Guo Huang¹, Hong Pu², Bo Gong³, He Lin¹, Shi Ma¹, Xuemei Chen⁴, Bo Long⁵, Guo Si⁵, Hua Yu¹, Li Jiang¹, Xingxiang Yang⁶, Yi Shi⁷, Zhenglin Yang⁸

Affiliations

¹ Sichuan Provincial Key Laboratory for Human Disease Gene Study and Institute of Laboratory Medicine, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.
² Department of Radiology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.
³ Sichuan Provincial Key Laboratory for Human Disease Gene Study and Institute of Laboratory Medicine, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China; Institute of Biology, Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, Chengdu, Sichuan, China.
⁴ Department of Infectious Disease, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.
⁵ Department of Infectious Disease, Sichuan Mianyang 404 Hospital, Mianyang, China.
⁶ Department of Infectious Disease, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China. Electronic address: 350686908@qq.com.
⁷ Sichuan Provincial Key Laboratory for Human Disease Gene Study and Institute of Laboratory Medicine, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China; Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences (2019RU026), Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, Sichuan, China. Electronic address: shiyi1614@126.com.
⁸ Sichuan Provincial Key Laboratory for Human Disease Gene Study and Institute of Laboratory Medicine, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China; Institute of Biology, Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, Chengdu, Sichuan, China; Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences (2019RU026), Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, Sichuan, China. Electronic address: zhenglin.yang@hsc.utah.edu.

PMID: 32114193
PMCID: PMC7129110
DOI: 10.1016/j.ijid.2020.02.050

Abstract

An ongoing outbreak of severe respiratory pneumonia associated with the 2019 novel coronavirus has recently emerged in China. Here we report the epidemiological, clinical, laboratory and radiological characteristics of 19 suspect cases. We compared the positive ratio of 2019-nCoV nucleic acid amplification test results from different samples including oropharyngeal swab, blood, urine and stool with 3 different fluorescent RT-PCR kits. Nine out of the 19 patients had 2019-nCoV infection detected using oropharyngeal swab samples, and the virus nucleic acid was also detected in eight of these nine patients using stool samples. None of positive results was identified in the blood and urine samples. These three different kits got the same result for each sample and the positive ratio of nucleic acid detection for 2019-nCoV was only 47.4% in the suspect patients. Therefore, it is possible that infected patients have been missed by using nucleic acid detection only. It might be better to make a diagnosis combining the computed tomography scans and nucleic acid detection.

Keywords: 2019 Novel coronavirus pneumonia; Clinical diagnosis; Nucleic acid amplification test.

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Figures

**Figure 1**
CT scans of the 2019-nCoV nucleic acid–detected positive patients. Increasing and multifocal ground-glass changes were visible. (A) Patient 1, January 30, 2020 (hospital day 2, illness day 5, A-1); February 1, 2020 (hospital day 4, illness day 7, A-2). (B) Patient 2, January 30, 2020 (hospital day 2, illness day 5, B-1); February 1, 2020 (hospital day 4, illness day 7, B-2). (C) Patient 3, January 30, 2020 (hospital day 1, illness day 6, C-1); February 1, 2020 (hospital day 3, illness day 8, C-2). (D) Suspected case 1, January 31, 2020 (hospital day 1, illness day 4, D-1); February 1, 2020 (hospital day 6, illness day 9, D-2). (E) Suspected case 3, February 1, 2020 (hospital day 1, illness day 6, E-1); February 5, 2020 (hospital day 5, illness day 10, E-2). (F) Suspected case 4, January 31, 2020 (hospital day 1, illness day 2, F-1); February 1, 2020 (hospital day 4, illness day 5, F-2).

See this image and copyright information in PMC

References

1. Cui J., Li F., Shi Z.L. Origin and evolution of pathogenic coronaviruses. Nat Rev Microbiol. 2019;17(3):181–192. - PMC - PubMed
1. Drosten C., Gunther S., Preiser W., van der Werf S., Brodt H.R., Becker S. Identification of a novel coronavirus in patients with severe acute respiratory syndrome. N Engl J Med. 2003;348(20):1967–1976. - PubMed
1. Fouchier R.A., Kuiken T., Schutten M., van Amerongen G., van Doornum G.J., van den Hoogen B.G. Aetiology: Koch’s postulates fulfilled for SARS virus. Nature. 2003;423(6937):240. - PMC - PubMed
1. To K.K.W., Yip C.C.Y., Lai C.Y.W., Wong C.K.H., Ho D.T.Y., Pang P.K.P. Saliva as a diagnostic specimen for testing respiratory virus by a point-of-care molecular assay: a diagnostic validity study. Clin Microbiol Infect. 2019;25(3):372–378. - PubMed
1. Wang C., Horby P.W., Hayden F.G., Gao G.F. A novel coronavirus outbreak of global health concern. Lancet. 2020;395(10223):470–473. - PMC - PubMed

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Comparison of different samples for 2019 novel coronavirus detection by nucleic acid amplification tests

Affiliations

Comparison of different samples for 2019 novel coronavirus detection by nucleic acid amplification tests

Authors

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Abstract

Figures

References

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