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. 2020 Mar 2;2020(3):100461.
doi: 10.1101/pdb.prot100461.

Recovery of DNA from Low-Melting-Temperature Agarose Gels: Organic Extraction

Michael R Green, Joseph Sambrook

Recovery of DNA from Low-Melting-Temperature Agarose Gels: Organic Extraction

Michael R Green et al. Cold Spring Harb Protoc. .

Abstract

In this protocol, DNA fragments are separated according to size by electrophoresis through low-melting-temperature agarose, and then recovered by melting the agarose and extracting with phenol:chloroform. The protocol works best for DNA fragments ranging in size from 0.5 to 5.0 kb. Yields of DNA fragments outside this range are usually lower, but often are sufficient for many purposes.

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