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Review
. 2020 Sep;11(5):e1593.
doi: 10.1002/wrna.1593. Epub 2020 Mar 3.

Transcription and splicing: A two-way street

Michael Tellier  1 , Isabella Maudlin  1 , Shona Murphy  1
Affiliations
Review

Transcription and splicing: A two-way street

Michael Tellier et al. Wiley Interdiscip Rev RNA. 2020 Sep.

Abstract

RNA synthesis by RNA polymerase II and RNA processing are closely coupled during the transcription cycle of protein-coding genes. This coupling affords opportunities for quality control and regulation of gene expression and the effects can go in both directions. For example, polymerase speed can affect splice site selection and splicing can increase transcription and affect the chromatin landscape. Here we review the many ways that transcription and splicing influence one another, including how splicing "talks back" to transcription. We will also place the connections between transcription and splicing in the context of other RNA processing events that define the exons that will make up the final mRNA. This article is categorized under: RNA Processing > Splicing Mechanisms RNA Processing > Splicing Regulation/Alternative Splicing.

Keywords: RNA polymerase II; RNA processing; exon definition; splicing.

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References

REFERENCES

    1. Adelman, K., & Lis, J. T. (2012). Promoter-proximal pausing of RNA polymerase II: Emerging roles in metazoans. Nature Reviews. Genetics, 13(10), 720-731. https://doi.org/10.1038/nrg3293
    1. Akhtar, J., Kreim, N., Marini, F., Mohana, G., Brune, D., Binder, H., & Roignant, J. Y. (2019). Promoter-proximal pausing mediated by the exon junction complex regulates splicing. Nature Communications, 10(1), 521. https://doi.org/10.1038/s41467-019-08381-0
    1. Alexander, R. D., Innocente, S. A., Barrass, J. D., & Beggs, J. D. (2010). Splicing-dependent RNA polymerase pausing in yeast. Molecular Cell, 40(4), 582-593. https://doi.org/10.1016/j.molcel.2010.11.005
    1. Ameur, A., Zaghlool, A., Halvardson, J., Wetterbom, A., Gyllensten, U., Cavelier, L., & Feuk, L. (2011). Total RNA sequencing reveals nascent transcription and widespread co-transcriptional splicing in the human brain. Nature Structural & Molecular Biology, 18(12), 1435-1440. https://doi.org/10.1038/nsmb.2143
    1. Amit, M., Donyo, M., Hollander, D., Goren, A., Kim, E., Gelfman, S., … Ast, G. (2012). Differential GC content between exons and introns establishes distinct strategies of splice-site recognition. Cell Reports, 1(5), 543-556. https://doi.org/10.1016/j.celrep.2012.03.013

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