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. 2020 Mar 4;10(1):3990.
doi: 10.1038/s41598-020-61074-3.

Sensitive determination of malondialdehyde in rat prostate by high performance liquid chromatography with fluorescence detection

Xiuli Dong  1 , Jiayuan Tang  1 , Xiangming Chen  2
Affiliations

Sensitive determination of malondialdehyde in rat prostate by high performance liquid chromatography with fluorescence detection

Xiuli Dong et al. Sci Rep. .

Abstract

An excellent pre-column fluorescent derivatization reagent N-acetylhydrazine acridone for the quantitative determination of malondialdehyde was synthesized. Malondialdehyde was derivatized at 80 °C for 30 min in the presence of trichloroacetic acid. The separation of the derivative was performed on an Agilent ZORBAX SB-C18 column in conjunction with gradient elution. The excitation and emission wavelengths were 370 nm and 420 nm, respectively. The developed method demonstrated good linear relationship in the range of 0.02 pmol to 2.5 pmol (r = 0.9998). The calculated limit of detection and limit of quantification were 2.5 fmol and 8.3 fmol, respectively. The analytical precisions of the method were in the range of 1.36-2.27% (intra-day) and 2.36-3.92% (inter-day) respectively. The method was sensitive, specific and simple. It was successfully implemented to analysis the malondialdehyde in rat prostate.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Synthesis route of AHAD.
Figure 2
Figure 2
Effect of catalyst (a), temperature (b), time (c) and amount of AHAD (d) on derivatization reaction.
Figure 3
Figure 3
Chromatogram of standard MDA (a) and MDA in rat prostate sample (b).
Figure 4
Figure 4
The calibration curve of MDA.
See this image and copyright information in PMC

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