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Comment
. 2020 May;17(5):536-538.
doi: 10.1038/s41423-020-0385-z. Epub 2020 Mar 4.

Novel antibody epitopes dominate the antigenicity of spike glycoprotein in SARS-CoV-2 compared to SARS-CoV

Ming Zheng  1 , Lun Song  2
Affiliations
Comment

Novel antibody epitopes dominate the antigenicity of spike glycoprotein in SARS-CoV-2 compared to SARS-CoV

Ming Zheng et al. Cell Mol Immunol. 2020 May.
No abstract available

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Antibody epitope analysis of spike proteins in MERS-CoV, SARS-CoV, and SARS-CoV-2. a Alignment and phylogenetic analysis of the amino acid sequences of spike proteins in SARS-CoV-2, MERS-CoV, and five representative SARS-CoVs (total sequence alignment in Supplementary Fig. 2). b Antibody epitope scores in spike proteins of MERS-CoV, SARS-CoV, and SARS-CoV-2; the grey dashed lines indicate the default threshold of antibody epitope scores. c Density plot of the distributions of antibody epitope scores in spike proteins of MERS-CoV, SARS-CoV, and SARS-CoV-2, with colors showing tail distribution probability, and the grey dashed lines representing the first, second, and third quartiles, respectively. The results were considered statistically significant when *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 using Kruskal–Wallis test. d Illustration of conserved and non-conserved sequences. e Density plot of the distributions of antibody epitope scores in conserved and non-conserved sequences in the comparison between spike proteins from SARS-CoV and SARS-CoV-2. The results were considered statistically significant when *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 using Wilcoxon’s signed-rank test. f The distributions of antibody epitope scores in conserved and non-conserved sequences in the comparison between spike proteins from MERS-CoV and SARS-CoV-2 using Wilcoxon’s signed-rank test. g Surface epitope accessibility scores in spike proteins of MERS-CoV, SARS-CoV, and SARS-CoV-2, the grey dashed lines indicate the default threshold of surface epitope accessibility scores. h The distributions of surface epitope accessibility scores in spike proteins of MERS-CoV, SARS-CoV, and SARS-CoV-2 using Kruskal–Wallis test. i The distributions of surface epitope accessibility scores in conserved and non-conserved sequences in the comparison between spike proteins from SARS-CoV and SARS-CoV-2 using Wilcoxon’s signed-rank test. j The distributions of surface epitope accessibility scores in conserved and non-conserved sequences in the comparison between spike proteins from MERS-CoV and SARS-CoV-2 using Wilcoxon’s signed-rank test. k Illustration of antibody epitope identification. l Venn plot of antibody epitopes in spike proteins of MERS-CoV, SARS-CoV, and SARS-CoV-2. m Illustration of analyzing intersected epitopes of unique, shared and public groups. n Plot of the number of intersected epitopes in spike proteins of MERS-CoV, SARS-CoV, and SARS-CoV-2. o Number of shared and unique epitopes from conserved, non-conserved, and the combination of conserved and non-conserved regions in the spike proteins of SARS-CoV and SARS-CoV-2. The color and size of cycles represent the number of epitopes, and the “×” represents no epitope could be found. p Structural model of the SARS-CoV-2 spike protein (yellow) in complex with its human cell receptor ACE2 (cyan). The model was superimposed using the structure of the SARS-CoV spike protein and ACE2 protein complex (PDB accession: 6ACJ) as template. The gray shadow represents the receptor-binding domain (RBD). q Number of epitopes located in RBD and non-RBD from conserved, non-conserved, and the combination of conserved and non-conserved regions in the SARS-CoV-2 spike protein. The color and size of cycles represent the number of epitopes, and the “×” represents no epitope could be found. r Plot of antibody epitope scores and surface epitope accessibility scores in the epitopes, each dot represent an epitope, the green dashed lines indicate the medians, respectively, the dot color represents the source of epitopes, the dot size represents the amino acid length of epitopes, the “*” represents the epitopes located in RBD, and the light green area shows the high-score epitopes. s Detail information of the high-score antibody epitopes from the SARS-CoV-2 spike protein
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References

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