Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Dec;22(12):1408-1414.
doi: 10.22038/IJBMS.2019.13988.

Effect of resveratrol on SNARE proteins expression and insulin resistance in skeletal muscle of diabetic rats

Azam Rezaei Farimani  1   2 , Mohammad Taghi Goodarzi  3 , Massoud Saidijam  3 , Reza Yadegarazari  4 , Sadegh Zarei  5 , Soheila Asadi  6
Affiliations

Effect of resveratrol on SNARE proteins expression and insulin resistance in skeletal muscle of diabetic rats

Azam Rezaei Farimani et al. Iran J Basic Med Sci. 2019 Dec.

Abstract

Objectives: Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex proteins are involved in membrane trafficking. The expression of isoforms of SNAP-23, syntaxin-4, and VAMP-2 is significantly done in skeletal muscles; they control GLUT4 trafficking. It is believed that type 2 diabetes could be caused by the modifications in the expression of SNARE complex proteins. The purpose of this study was to evaluate the effect of resveratrol on the expression of these proteins in type 2 diabetes.

Materials and methods: Forty male Wistar rats were selected. Streptozotocin and nicotinamide were applied for the induction of type 2 diabetes. The animals were divided into five groups. Healthy and diabetic groups were set as control; resveratrol (1, 5, and 10 mg/kg body weight) was applied to treat the three groups of diabetic rats for 30 days. Real-time qRT-PCR was applied to evaluate the expression of SNARE complex proteins.

Results: There is a link between diabetes and insulin resistance and up-regulation of SNARE proteins expression. Resveratrol improved hyperglycemia and insulin resistance along with a non-significant reduction in the expression of SNARE proteins.

Conclusion: Increased expression of SNARE proteins was possibly a compensatory mechanism in response to insulin resistance in the skeletal muscles of diabetic rats. Resveratrol non-significantly reduced the expression of SNARE proteins by enhancing insulin sensitivity, where this effect was dose-dependent. Thus, higher doses of resveratrol and longer intervention periods could probably be more effective. Another molecular mechanism of the anti-diabetic properties of resveratrol was identified with an effect on the expression of SNARE proteins.

Keywords: Resveratrol; SNAP-23; SNARE complex proteins; Syntaxin-4; Type 2 diabetes; VAMP-2.

PubMed Disclaimer

Conflict of interest statement

The authors declare that there are no conflicts of interest.

References

    1. Hameed I, Masoodi SR, Mir SA, Nabi M, Ghazanfar K, Ganai BA. Type 2 diabetes mellitus: from a metabolic disorder to an inflammatory condition. World J Diabetes. 2015;6:598–612. - PMC - PubMed
    1. Huang S, Czech MP. The GLUT4 glucose transporter. Cell Metab. 2007;5:237–252. - PubMed
    1. Rea S, James DE. Moving GLUT4: the biogenesis and trafficking of GLUT4 storage vesicles. Diabetes. 1997;46:1667–1677. - PubMed
    1. Stöckli J, Fazakerley DJ, James DE. GLUT4 exocytosis. J Cell Sci. 2011;124:4147–4159. - PMC - PubMed
    1. Duman JG, Forte JG. What is the role of SNARE proteins in membrane fusion? Am J Physiol Cell Physiol. 2003;285:C237–C249. - PubMed

LinkOut - more resources