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. 2020 Feb 19:8:14.
doi: 10.3389/fchem.2020.00014. eCollection 2020.

A Fluorescent and Colorimetric Chemosensor for Hg2+ Based on Rhodamine 6G With a Two-Step Reaction Mechanism

Cui-Bing Bai  1   2 , Wei-Gang Wang  1 , Jie Zhang  1 , Chang Wang  1   2 , Rui Qiao  1   2 , Biao Wei  1   2 , Lin Zhang  1   2 , Shui-Sheng Chen  1   2 , Song Yang  1   2
Affiliations

A Fluorescent and Colorimetric Chemosensor for Hg2+ Based on Rhodamine 6G With a Two-Step Reaction Mechanism

Cui-Bing Bai et al. Front Chem. .

Abstract

A fluorescent and colorimetric chemosensor L based on rhodamine 6G was designed, synthesized, and characterized. Based on a two-step reaction, the chemosensor L effectively recognized Hg2+. The interaction between the chemosensor and Hg2+ was confirmed by ultraviolet-visible spectrophotometry, fluorescence spectroscopy, electrospray ionization-mass spectrometry, Fourier-transform infrared spectroscopy, and frontier molecular orbital calculations. The chemosensor L was also incorporated into test strips and silica gel plates, which demonstrated good selectivity and high sensitivity for Hg2+.

Keywords: Hg2+; chemosensor; rhodamine 6G; silica gel plates; test strips.

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Figures

Figure 1
Figure 1
Strategies for the chemosensing of Hg2+. Type A: The coordination between ligand and Hg2+. Type B: The desulfurization between ligand and Hg2+. Type C: The two-step reaction mechanism.
Figure 2
Figure 2
Synthesis of chemosensor L. (A) Compound A. (B) Compound B. (C) Compound C.
Figure 3
Figure 3
(A) Absorption spectra of L (1.0 × 10−5 M) in the presence of various metal ions in HEPES buffer. (B) Photographs of L (1.0 × 10−5 M) in the presence of various metal ions.
Figure 4
Figure 4
(A) Fluorescence spectra of L (1.0 × 10−5 M) in the presence of various metal ions in HEPES buffer (λex = 526 nm). (B) Photographs of chemosensor L (1.0 × 10−5 M) in the presence of various metal ions under the hand-held ultraviolet lamp.
Figure 5
Figure 5
Proposed mechanism for Hg2+ detection by chemosensor L.
Figure 6
Figure 6
(A) Fluorescence spectra of L (1.0 × 10−5 M) in the presence of different concentrations of Hg2+ in HEPES buffer (λex = 526 nm). (B) Plot of fluorescence intensity at 550 nm vs. Hg2+ concentration in the range of 0.1–3.0 equiv.
Figure 7
Figure 7
Molecular orbital plots (VESTA software) of the HOMOs and LUMOs of L, LO, and LO–Hg2+.
Figure 8
Figure 8
Photographs of test strips and silica gel plates.
See this image and copyright information in PMC

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